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Titolo:
Glutamine synthetase and its isoforms in rice spikelets and rachis during grain development
Autore:
Zhang, CF; Peng, SB; Bennett, J;
Indirizzi:
Int Rice Res Inst, Agron Plant Physiol & Agroecol Div, Makati City 1271, Philippines Int Rice Res Inst Makati City Philippines 1271 ti City 1271, Philippines Int Rice Res Inst, Plant Breeding Genet & Biochem Div, Makati City 1271, Philippines Int Rice Res Inst Makati City Philippines 1271 ti City 1271, Philippines Wuhan Univ, Sch Life Sci, Wuhan 430072, Peoples R China Wuhan Univ WuhanPeoples R China 430072 i, Wuhan 430072, Peoples R China
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 2, volume: 156, anno: 2000,
pagine: 230 - 233
SICI:
0176-1617(200002)156:2<230:GSAIII>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL SENESCENCE; ENZYME-ACTIVITIES; FLAG LEAF; BARLEY; NITROGEN; LEAVES; MAIZE; PHOTOSYNTHESIS; CHLOROPLAST; METABOLISM;
Keywords:
glutamine synthetase; isoform; leaf; rachis; rice; spikelet;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Peng, SB Int Rice Res Inst, Agron Plant Physiol & Agroecol Div, POB 3127,MCPO, Makati City 1271, Philippines Int Rice Res Inst POB 3127,MCPO Makati City Philippines 1271 nes
Citazione:
C.F. Zhang et al., "Glutamine synthetase and its isoforms in rice spikelets and rachis during grain development", J PLANT PHY, 156(2), 2000, pp. 230-233

Abstract

Glutamine synthetase (GS) and its isoforms have been studied extensively in rice (Oryza sativa L.) leaves, but little is known about GS in the spikelets and rachis of rice panicles. In this study, GS activity and its isoforms were determined in the spikelets, rachis, and flag leaves during grain development. The GS activities per unit fresh weight of these tissues reachedmaximum levers around the milk stage of the endosperm and declined thereafter as grain filling progressed. Flag leaves had 5-fold higher GS activity per unit fresh weight than rachis and 7-fold higher than spikelets at the milk stage. Native-PAGE and activity staining indicated that there were two GS isoforms in the rachis and they were the same as the cytosolic form (GS1) and the chloroplastic form (GS2) in leaves, respectively. The GS2 was thedominant isoform in flag leaves, whereas GSI aas the dominant isoform in the rachis. However, only GSI activity was detectable in spikelets includinghull and kernel. The lack of GS2 activity in the spikelets was consistent throughout the entire grain development stage and was confirmed by protein immunoblotting.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:25:24