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Titolo:
The neuronal monoamine transporter VMAT2 is regulated by the trimeric GTPase Go(2)
Autore:
Holtje, M; von Jagow, B; Pahner, I; Lautenschlager, M; Hortnagl, H; Nurnberg, B; Jahn, R; Ahnert-Hilger, G;
Indirizzi:
Humboldt Univ, Charite, Inst Anat, D-10115 Berlin, Germany Humboldt Univ Berlin Germany D-10115 Inst Anat, D-10115 Berlin, Germany Humboldt Univ, Charite, Inst Pharmakol, D-10115 Berlin, Germany Humboldt Univ Berlin Germany D-10115 Pharmakol, D-10115 Berlin, Germany Humboldt Univ, Charite, Neurol Klin, D-10115 Berlin, Germany Humboldt Univ Berlin Germany D-10115 eurol Klin, D-10115 Berlin, Germany Free Univ Berlin, Inst Pharmakol, D-14195 Berlin, Germany Free Univ Berlin Berlin Germany D-14195 armakol, D-14195 Berlin, Germany Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany Max Planck Inst Biophys Chem Gottingen Germany D-37077 ottingen, Germany
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 6, volume: 20, anno: 2000,
pagine: 2131 - 2141
SICI:
0270-6474(20000315)20:6<2131:TNMTVI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; SMALL SYNAPTIC VESICLES; DENSE CORE VESICLES; QUANTAL SIZE; PARKINSONS-DISEASE; SUBSTANTIA-NIGRA; CEREBRAL-CORTEX; DENTATE GYRUS; AMINO-ACIDS; PC12 CELLS;
Keywords:
VMAT2; Go(2); neuroendocrine cells; neurons; transmitter uptake; vesicular plasticity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Ahnert-Hilger, G Humboldt Univ, Charite, Inst Anat, Philippstr 12, D-10115Berlin, Germany Humboldt Univ Philippstr 12 Berlin Germany D-10115 rmany
Citazione:
M. Holtje et al., "The neuronal monoamine transporter VMAT2 is regulated by the trimeric GTPase Go(2)", J NEUROSC, 20(6), 2000, pp. 2131-2141

Abstract

Monoamines such as noradrenaline and serotonin are stored in secretory vesicles and released by exocytosis. Two related monoamine transporters, VMAT1and VMAT2, mediate vesicular transmitter uptake. Previously we have reported that in the rat pheochromocytoma cell line PC 12 VMAT1, localized to peptide-containing secretory granules, is controlled by the heterotrimeric G-protein Go(2). We now show that in BON cells, a human serotonergic neuroendocrine cell line derived from a pancreatic tumor expressing both transporters on large, dense-core vesicles, VMAT2 is even more sensitive to G-protein regulation than VMAT1. The activity of both transporters is only downregulated by G alpha o(2), whereas comparable concentrations of G alpha o(1) are without effect. In serotonergic raphe neurons in primary culture VMAT2 is also downregulated by pertussis toxin-sensitive Go(2). By electron microscopic analysis from prefrontal cortex we show that VMAT2 and G alpha o(2) associate preferentially to locally recycling small synaptic vesicles in serotonergic terminals. In addition, Go(2)-dependent modulation of VMAT2 also works when using a crude synaptic vesicle preparation from this brain area. Weconclude that regulation of monoamine uptake by the heterotrimeric G proteins is a general feature of monoaminergic neurons that controls the contentof both large, dense-core and small synaptic vesicles.

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Documento generato il 27/09/20 alle ore 01:00:37