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Titolo:
TNF-alpha, IL-4, and IFN-gamma regulate differential expression of P and E-selectin expression by porcine aortic endothelial cells
Autore:
Stocker, CJ; Sugars, KL; Harari, OA; Landis, RC; Morley, BJ; Haskard, DO;
Indirizzi:
Hammersmith Hosp, British Heart Fdn, Cardiovasc Med Unit, NHLI,Imperial Coll,Sch Med, London W12 0NN, England Hammersmith Hosp London England W12 0NN Sch Med, London W12 0NN, England Hammersmith Hosp, Dept Med, Imperial Coll, Sch Med, London W12 0NN, England Hammersmith Hosp London England W12 0NN Sch Med, London W12 0NN, England
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 6, volume: 164, anno: 2000,
pagine: 3309 - 3315
SICI:
0022-1767(20000315)164:6<3309:TIAIRD>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRANULE MEMBRANE-PROTEIN; TUMOR-NECROSIS-FACTOR; WEIBEL-PALADE BODIES; ADHESION MOLECULE; MONOCLONAL-ANTIBODY; CLONING; GMP-140; FAMILY; RECRUITMENT; LYMPHOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Haskard, DO Hammersmith Hosp, British Heart Fdn, Cardiovasc Med Unit, NHLI,Imperial Coll,Sch Med, London W12 0NN, England Hammersmith Hosp London England W12 0NN don W12 0NN, England
Citazione:
C.J. Stocker et al., "TNF-alpha, IL-4, and IFN-gamma regulate differential expression of P and E-selectin expression by porcine aortic endothelial cells", J IMMUNOL, 164(6), 2000, pp. 3309-3315

Abstract

P- and E-selectin are surface glycoproteins that mediate leukocyte rollingon the surface of endothelium in inflammation. We have cloned porcine P-selectin cDNA and generated a mAb, 12C5, with which to examine P-selectin expression by porcine aortic endothelial cells (PAEC) in comparison with that of E selectin. Basal expression by PAEC of P-selectin was greater than thatof E-selectin, whereas E-selectin expression was more prominently enhancedthan that of P-selectin by stimulation with TNF-alpha or IL-1 alpha, Both human or porcine IL-4 led to an increase in P-selectin expression, with kinetics that were delayed compared with those seen following stimulation withTNF-alpha or IL-1 alpha, but IL-4 did not stimulate expression of E-selectin, When cells were stimulated with TNF-alpha in the presence of IL-4, we observed enhanced P-selectin expression with a parallel reduction in E-selectin expression. Finally, the increase in P-selectin expression due to humanIL-4 was reduced in the presence of porcine but not human IFN-gamma, Theseobservations show that E-selectin and P-selectin expression are differentially regulated in PAEC, and that IL-4 leads to a shift in the relative surface density of the two molecules toward P-selectin. The ability of porcine IFN-gamma to inhibit IL-il-induced P-selectin expression suggests that the balance between Th1 and Th2 cytokine production may determine the relative densities of the two selectins in chronic immune-mediated inflammation. Because the increased expression of F-selectin induced by human IL-4 was not inhibited by human IFN-gamma, this balance may he shifted toward P-selectin expression in porcine xenografts infiltrated by human lymphocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 12:06:25