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Titolo:
Direct high-performance liquid chromatographic determination of (R)- and (S)-propranolol in rat microdialysate using on-line column switching procedures
Autore:
Mislanova, C; Stefancova, A; Oravcova, J; Horecky, J; Trnovec, T; Lindner, W;
Indirizzi:
Inst Prevent & Clin Med, SK-83301 Bratislava, Slovakia Inst Prevent & ClinMed Bratislava Slovakia SK-83301 ratislava, Slovakia Univ Vienna, Inst Analyt Chem, A-1090 Vienna, Austria Univ Vienna ViennaAustria A-1090 st Analyt Chem, A-1090 Vienna, Austria
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 739, anno: 2000,
pagine: 151 - 161
SICI:
1387-2273(20000228)739:1<151:DHLCDO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHIRAL STATIONARY-PHASE; PLASMA-PROTEIN BINDING; DIRECT-INJECTION; PROPRANOLOL ENANTIOMERS; ASSAY; SILICA; SEPARATION; SAMPLES; ONLINE; TISSUE;
Keywords:
column switching; propranolol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Mislanova, C Inst Prevent & Clin Med, Limbova 14, SK-83301 Bratislava, Slovakia Inst Prevent & Clin Med Limbova 14 Bratislava Slovakia SK-83301
Citazione:
C. Mislanova et al., "Direct high-performance liquid chromatographic determination of (R)- and (S)-propranolol in rat microdialysate using on-line column switching procedures", J CHROMAT B, 739(1), 2000, pp. 151-161

Abstract

Two different column-switching HPLC systems (CSWs), employing restricted access material for initial pretreatment of biological samples, were developed for the determination of propranolol enantiomers in microdialysate. CSW 1 was a single-pump set-up based on an initial sample clean-up step with a RP-18 ADS precolumn coupled with an ovomucoid analytical column for direct drug enantioseparation. For the two-pump column set-up (CSW 2), a teicoplanin analytical column was applied for the enantioselective assay after initial sample pretreatment using a RP-8 ADS precolumn. The inter-day precision of the CSW 1 ranged from 0.5 to 5.1% for (R)-propranolol and from 5.1 to 10.5% for (S)-propranolol, The limit of detection (LOD) was set at 10 ng/ml and 15 ng/ml for (R)- and (S)-propranolol, respectively. Inter-day relative standard deviation values of the CSW 2 ranged from 1.1 to 9.9% for (R)-propranolol and from 1.3 to 9.6% for (S)-propranolol. The LOD of the method was3.0 ng/ml for (R)-propranolol and 2.5 ng/ml for (S)-propranolol. Both approaches were successfully applied for stereoselective monitoring of unbound propranolol levels in rat microdialysates. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:40:15