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Titolo:
The differential time-course of extracellular-regulated kinase activity correlates with the macrophage response toward proliferation or activation
Autore:
Valledor, AF; Comalada, M; Xaus, J; Celada, A;
Indirizzi:
Univ Barcelona, Fac Biol, Dept Fisiol Biol Macrofag, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 acrofag, E-08028 Barcelona, Spain Univ Barcelona, Fundacio August Pi & Sunyer, Dept Fisiol Biol Macrofag, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 acrofag, E-08028 Barcelona, Spain
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 10, volume: 275, anno: 2000,
pagine: 7403 - 7409
SICI:
0021-9258(20000310)275:10<7403:TDTOEK>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CSF-1 SIGNAL-TRANSDUCTION; EPIDERMAL GROWTH-FACTOR; TUMOR-NECROSIS-FACTOR; PROTEIN-KINASE; INDEPENDENT PATHWAYS; PRIMARY CULTURES; RAT HEPATOCYTES; GENE-EXPRESSION; DNA-SYNTHESIS; TNF-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Celada, A Univ Barcelona, Fac Biol, Dept Fisiol Biol Macrofag, Campus Bellvitge,Av Diagonal 645, E-08028 Barcelona, Spain Univ Barcelona Campus Bellvitge,Av Diagonal 645 Barcelona Spain E-08028
Citazione:
A.F. Valledor et al., "The differential time-course of extracellular-regulated kinase activity correlates with the macrophage response toward proliferation or activation", J BIOL CHEM, 275(10), 2000, pp. 7403-7409

Abstract

Bone marrow-derived macrophages proliferate in response to specific growthfactors, including macrophage colony-stimulating factor (M-CSF), When stimulated with activating factors, such as lipopolysaccharide (LPS), macrophages stop proliferating and produce proinflammatory cytokines, Although triggering opposed responses, both M-CSF and LPS induce the activation of extracellular-regulated kinases (ERKs) 1 and 2. However, the time-course of ERK activation is different; maximal activation by M-CSF and LPS occurred after 5 and 15 min of stimulation, respectively. Granulocyte/macrophage colony-stimulating factor, interleukin 3, and TPA, all of which induced macrophage proliferation, also induced ERK activity, which was maximal at 5 min poststimulation, The use of PD98059, which specifically blocks ERK 1 and 2 activation, demonstrated that ERK activity was necessary for macrophage proliferation in response to these factors. The treatment with phosphatidylcholine-specific phospholipase C (PC-PLC) inhibited macrophage proliferation, inducedthe expression of cytokines, and triggered a pattern of ERK activation equivalent to that induced by LPS, Moreover, PD98059 inhibited the expression of cytokines induced by LPS or PC-PLC, thus suggesting that ERK activity isalso required for macrophage activation by these two agents. Activation ofthe JNK pathway did not discriminate between proliferative and activating stimuli. In conclusion, our results allow to correlate the differences in the time-course of ERK activity with the macrophase response toward proliferation or activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 17:58:30