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Titolo:
Molecular cloning and expression of mouse GD1 alpha/GT1a alpha/GQ1b alpha synthase (ST6GalNAc VI) gene
Autore:
Okajima, T; Chen, HH; Ito, H; Kiso, M; Tai, T; Furukawa, K; Urano, T; Furukawa, K;
Indirizzi:
Nagoya Univ, Sch Med, Dept Biochem 2, Nagoya, Aichi 4660065, Japan Nagoya Univ Nagoya Aichi Japan 4660065 em 2, Nagoya, Aichi 4660065, Japan Nagoya Univ, Sch Med, Dept Surg 2, Nagoya, Aichi 4660065, Japan Nagoya Univ Nagoya Aichi Japan 4660065 rg 2, Nagoya, Aichi 4660065, Japan Gifu Univ, Fac Agr, Dept Appl Bioorgan Chem, Gifu 5011193, Japan Gifu Univ Gifu Japan 5011193 ept Appl Bioorgan Chem, Gifu 5011193, Japan Tokyo Metropolitan Inst Med Sci, Dept Tumor Immunol, Bunkyo Ku, Tokyo 1138613, Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 1138613 Tokyo 1138613, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 10, volume: 275, anno: 2000,
pagine: 6717 - 6723
SICI:
0021-9258(20000310)275:10<6717:MCAEOM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURON-SPECIFIC GANGLIOSIDE; GALNAC ALPHA-2,6-SIALYLTRANSFERASE; CELLS; GQ1B-ALPHA; GD1-ALPHA; GLYCOLIPIDS; ANTIBODIES; GT1A-ALPHA; ANTIGENS; MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Nagoya Univ, Sch Med, Dept Biochem 2, Nagoya, Aichi 4660065, Japan Nagoya Univ Nagoya Aichi Japan 4660065 , Aichi 4660065, Japan
Citazione:
T. Okajima et al., "Molecular cloning and expression of mouse GD1 alpha/GT1a alpha/GQ1b alpha synthase (ST6GalNAc VI) gene", J BIOL CHEM, 275(10), 2000, pp. 6717-6723

Abstract

A novel member of the mouse CMP-NeuAc:beta-N-acetyl-galactosaminide alpha 2,6-sialyltransferase (ST6GalNAc) subfamily, designated ST6GalNAc VI, was identified by BLAST analysis of expressed sequence tags. The sequence of thecDNA clone of ST6GalNAc VI encoded a type II membrane protein with 43 amino acids composing the cytoplasmic domain, 21 amino acids composing the transmembrane region, and 269 amino acids composing the catalytic domain. The predicted amino acid sequence showed homology to the previously cloned ST6GalNAc III, IV, and V, with common amino acid sequences in sialyl motif L andS among these four enzymes. A fusion protein with protein A and extracts from L cells transfected with ST6GalNAc VI in an expression vector showed enzyme activity of alpha 2,6-sialyltransferase for GM1b, GT1b, and GD1a but not toward glycoproteins. Thin layer chromatography-immunostaining revealed that the products were GD1 alpha, GQ1b alpha, and GT1a alpha. Northern blotting revealed that this gene was expressed in a wide range of mouse tissuessuch as colon, liver, heart, spleen, and brain. It is concluded that this enzyme is a novel sialyltransferase involved in the synthesis of alpha-series gangliosides in the nervous tissues and many other tissues.

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Documento generato il 23/01/21 alle ore 03:18:10