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Titolo:
Expression of fos and jun proto-oncogenes in benign versus malignant humanuterine tissue
Autore:
Nephew, KP; Choi, CM; Polek, TC; McBride, R; Bigsby, RM; Khan, SA; Husseinzadeh, N;
Indirizzi:
Univ Cincinnati, Coll Med, Dept Obstet & Gynecol, Div Gynecol Oncol, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 l Oncol, Cincinnati, OH 45267 USA Univ Cincinnati, Coll Med, Dept Anat & Cell Biol, Cincinnati, OH 45267 USAUniv Cincinnati Cincinnati OH USA 45267 ll Biol, Cincinnati, OH 45267 USA Indiana Univ, Sch Med, Dept Obstet & Gynecol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ynecol, Indianapolis, IN 46202 USA
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 3, volume: 76, anno: 2000,
pagine: 388 - 396
SICI:
0090-8258(200003)76:3<388:EOFAJP>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; ESTROGEN-INDUCED EXPRESSION; IMMEDIATE-EARLY GENES; RAT UTERUS; C-FOS; ENDOMETRIAL CANCER; PROTOONCOGENE; ACTIVATION; MYC; TRANSCRIPTION;
Keywords:
uterus; endometrial cancer; proto-oncogenes; fos; jun;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Husseinzadeh, N Univ Cincinnati, Coll Med, Dept Obstet & Gynecol, Div Gynecol Oncol, 231 Bethesda Ave, Cincinnati, OH 45267 USA Univ Cincinnati 231 Bethesda Ave Cincinnati OH USA 45267 A
Citazione:
K.P. Nephew et al., "Expression of fos and jun proto-oncogenes in benign versus malignant humanuterine tissue", GYNECOL ONC, 76(3), 2000, pp. 388-396

Abstract

Objective. The objective of this study was to evaluate expression of fos and jun proto-oncogenes in benign human uterine tissue compared with malignant uterine tissue. Methods. Forty-two endometrial tissue specimens were obtained at the time of hysterectomy. Tissue samples from different phases of the menstrual cycle and from postmenopausal patients were stained using immunohistochemical methods to detect Fos and Jun proteins, estrogen and progesterone receptor status, and Ki67 (detects a nuclear antigen associated with proliferating cells). Tissue was examined microscopically for nuclear staining in endometrial epithelium and stroma. The endometrium was based on the patient's last menstrual period, pathologic dating, and proliferative versus nonproliferative status as determined by Ki67. Benign and malignant specimens were subjected to Northern blot analysis to evaluate levels of expression of c-fos, c-jun, and jun-B mRNA. The pattern of c-fos mRNA expression in malignant samples was further evaluated using in situ hybridization. Results. In proliferative, secretory, postmenopausal, and progesterone-influenced, uterine specimens immunohistochemically stained and examined, the endometrial and stromal nuclei stained for both Fos and Jun in varying intensities. However, no pattern was found in the variation of intensity according to the phase of the endometrium. Similarly, in malignant and benign endometrial tissue examined by Northern blot and in situ hybridization analyses, expression of proto-oncogene mRNAs was readily detectable, but no statistical correlation between type of tissue examined, grade of adenocarcinoma,and stage of endometrial cancer was found in this study. Conclusions. In rodent models, control of uterine cell proliferation is related to change in expression of fos and jun protooncogenes. Our results indicate that hormonal control is likely to be different in human endometriumand probably involves genes other than the proto-oncogenes under study. Expression of Fos and Jun do not correlate with endometrial cancer stage and grade. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:14:24