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Titolo:
Possible involvement of the locus coeruleus in inhibition by prostanoid EP3 receptor-selective agonists of morphine withdrawal syndrome in rats
Autore:
Nakagawa, T; Masuda, T; Watanabe, T; Minami, M; Satoh, M;
Indirizzi:
Kyoto Univ, Fac Pharmaceut Sci, Dept Mol Pharmacol, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Dept Mol Pharmacol, Kyoto 6068501, Japan
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 3, volume: 390, anno: 2000,
pagine: 257 - 266
SICI:
0014-2999(20000303)390:3<257:PIOTLC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NALOXONE-PRECIPITATED WITHDRAWAL; IN-SITU HYBRIDIZATION; OPIATE WITHDRAWAL; C-FOS; NERVOUS-SYSTEM; MESSENGER-RNA; ABSTINENCE SYNDROME; PROSTAGLANDIN E(2); ADENYLATE-CYCLASE; GENE-EXPRESSION;
Keywords:
morphine withdrawal; prostaglandin; prostanoid EP3 receptor; mu-opioid receptor; c-fos; locus coeruleus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Satoh, M Kyoto Univ, Fac Pharmaceut Sci, Dept Mol Pharmacol, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Pharmacol, Kyoto 6068501, Japan
Citazione:
T. Nakagawa et al., "Possible involvement of the locus coeruleus in inhibition by prostanoid EP3 receptor-selective agonists of morphine withdrawal syndrome in rats", EUR J PHARM, 390(3), 2000, pp. 257-266

Abstract

We examined the mechanism of the inhibitory effect of prostanoid EP3 receptor agonists on naloxone-precipitated withdrawal syndrome in morphine-dependent rats. Rats were rendered morphine dependent by subcutaneous (s.c.) implantation of two pellets containing 75 mg morphine for 5 days. Morphine withdrawal syndrome was precipitated by i.p. injection of naloxone (3 mg/kg). Intracerebroventricular (i.c.v.) administration of (+/-)-15 alpha-hydroxy-9-oxo-16-phenoxy-17,18,19,20-tetranorprost-13-trans-enoic acid(M&B28,767: prostanoid EP3 receptor agonist) or sulprostone (prostanoid EP1/EP3 receptor agonist) significantly suppressed many withdrawal signs. Northern blotting and in situ hybridization studies revealed that i.c.v. administration of M&B28,767 (1 pg/rat) attenuated the elevation of c-fos mRNA during naloxone-precipitated withdrawal in many brain regions, including the cerebral cortex, thalamus, hypothalamus and locus coeruleus. Double in situ hybridization analysis revealed that in the locus coeruleus most of the tyrosine hydroxylase mRNA-positive neurons expressed mu-opioid receptor mRNA and more than half of these neurons were positive for prostanoid EP3 receptor mRNA. These results indicate that the suppression by prostanoid EP3 receptor agonists of naloxone-precipitated morphine withdrawal syndrome can be attributed to the inhibition of neuronal activity in several brain regions, including the locus coeruleus, the largest source of central noradrenergic neurons. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 21:07:29