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Titolo:
Isolation and properties of a cellulosome-type multienzyme complex of the thermophilic Bacteroides sp strain P-1
Autore:
Ponpium, P; Ratanakhanokchai, K; Kyu, KL;
Indirizzi:
King Mongkuts Univ Technol Thonburi, Sch Bioresources & Technol, Bangkok, Thailand King Mongkuts Univ Technol Thonburi Bangkok Thailand Bangkok, Thailand
Titolo Testata:
ENZYME AND MICROBIAL TECHNOLOGY
fascicolo: 5-6, volume: 26, anno: 2000,
pagine: 459 - 465
SICI:
0141-0229(200003)26:5-6<459:IAPOAC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLOSTRIDIUM-THERMOCELLUM; CELLULASE; BINDING; ORGANIZATION; DEGRADATION; ENZYMES;
Keywords:
cellulosome; multienzyme complex; Bacteroides sp strain P-1; cellulase (avicelase and carboxymethyl cellulase) and xylanase; cellulose-binding ability; affinity chromatography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Ratanakhanokchai, K King Mongkuts Univ Technol Thonburi, Sch Bioresources & Technol, Bangkok, Thailand King Mongkuts Univ Technol Thonburi Bangkok Thailand
Citazione:
P. Ponpium et al., "Isolation and properties of a cellulosome-type multienzyme complex of the thermophilic Bacteroides sp strain P-1", ENZYME MICR, 26(5-6), 2000, pp. 459-465

Abstract

The extracellular form of cellulosome-type multienzyme complex of thermophilic Bacteroides sp. strain P-l which was isolated from the anaerobic digester, is described. Multienzyme complex was isolated from the culture supernatant by an adsorption-desorption affinity chromatography on microcrystalline cellulose. The isolated multienzyme complex was found to form a complex that exhibited a high molecular weight (estimated at more than 1400 kDa) and was quite stable; requiring strong denaturing condition for dissociation. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate resolved multienzyme complex into at least 12 subunits with the molecular weight range of 49 to 209 kDa, respectively. The isolated: multienzyme complex showed cellulose-binding ability, cellulase and-xylanase activities and effected the hydrolysis of crystalline cellulose and lignocellulosic materials in the form of corncob, corn hull, rice straw, and sugarcane bagasse. (C) 2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 08:11:31