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Titolo:
Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy
Autore:
Schutz, GJ; Kada, G; Pastushenko, VP; Schindler, H;
Indirizzi:
Univ Linz, Inst Biophys, A-4040 Linz, Austria Univ Linz Linz Austria A-4040 v Linz, Inst Biophys, A-4040 Linz, Austria
Titolo Testata:
EMBO JOURNAL
fascicolo: 5, volume: 19, anno: 2000,
pagine: 892 - 901
SICI:
0261-4189(20000301)19:5<892:POLMIA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GPI-ANCHORED PROTEINS; SCANNING OPTICAL MICROSCOPY; PLASMA-MEMBRANE; ANOMALOUS DIFFUSION; SIGNAL-TRANSDUCTION; LATERAL DIFFUSION; EPITHELIAL-CELLS; MODEL MEMBRANES; LIVING CELLS; CAVEOLAE;
Keywords:
fluorescence imaging; human coronary artery smooth muscle cell; lipid rafts; liquid-ordered lipid phase; single molecule diffusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Schindler, H Univ Linz, Inst Biophys, A-4040 Linz, Austria Univ Linz Linz Austria A-4040 Biophys, A-4040 Linz, Austria
Citazione:
G.J. Schutz et al., "Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy", EMBO J, 19(5), 2000, pp. 892-901

Abstract

The lateral motion of single fluorescence labeled Lipid molecules was imaged in native cell membranes on a millisecond time scale and with positionalaccuracy of similar to 50 nm, using 'single dye tracing'. This first application of single molecule microscopy to living cells rendered possible the direct observation of lipid-specific membrane domains. These domains were sensed by a lipid probe with saturated acyl chains as small areas in a liquid-ordered phase: the probe showed confined but fast diffusion, with high partitioning (similar to 100-fold) and long residence time (similar to 13 s),The analogous probe with mono-unsaturated chains diffused predominantly unconfined within the membrane. With similar to 15 saturated probes per domain, the locations, sizes, shapes and motions of individual domains became clearly visible. Domains had a size of 0.7 mu m (0.2-2 mu m), covering similar to 13% of total membrane area. Both the liquid-ordered phase characteristics and the sizes of domains match properties of membrane fractions described as detergent-resistant membranes (DRMs), strongly suggesting that the domains seen are the in vivo correlate of DRMs and thus may be identified as lipid rafts.

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Documento generato il 20/09/20 alle ore 08:40:28