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Titolo:
Non-cardiac nucleic acid composition and protein synthesis rates in hypertension: studies on the spontaneously hypertensive rat (SHR) model
Autore:
Patel, VB; Richardson, PJ; Preedy, VR;
Indirizzi:
Univ London Kings Coll, Guys Kings & St Thomas Sch Med, Dept Clin Biochem,London SE5 9PJ, England Univ London Kings Coll London England SE5 9PJ em,London SE5 9PJ, England Univ London Kings Coll, Guys Kings & St Thomas Sch Med, Dept Cardiol, London SE5 9PJ, England Univ London Kings Coll London England SE5 9PJ l, London SE5 9PJ, England Univ London Kings Coll, Dept Nutr & Dietet, London SE1 8WA, England Univ London Kings Coll London England SE1 8WA t, London SE1 8WA, England
Titolo Testata:
CLINICA CHIMICA ACTA
fascicolo: 1-2, volume: 293, anno: 2000,
pagine: 167 - 179
SICI:
0009-8981(200003)293:1-2<167:NNACAP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; AORTIC CONSTRICTION; SKELETAL-MUSCLE; SURGICAL STRESS; NUTRIENT INTAKE; BLOOD-PRESSURE; WKY RATS; ETHANOL; HEART; CONTRACTILE;
Keywords:
heart; hypertrophy; SHR; protein synthesis; liver; bone; skin; muscle; kidney; brain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Patel, VB Wake Forest Univ, Sch Med, Dept Biochem, Med Ctr Blvd, Winston Salem, NC 27157 USA Wake Forest Univ Med Ctr Blvd Winston Salem NC USA 27157157 USA
Citazione:
V.B. Patel et al., "Non-cardiac nucleic acid composition and protein synthesis rates in hypertension: studies on the spontaneously hypertensive rat (SHR) model", CLIN CHIM A, 293(1-2), 2000, pp. 167-179

Abstract

Various studies have shown the involvement of extracardiac tissues in hypertension, including the hepato-intestinal tract, musculo-skeletal system, skin, and the kidney. It was our hypothesis that these perturbations in non-cardiac tissues would also include alterations in protein metabolism. Thus,the reported differences in soleus contractile protein composition may he related to changes in muscle protein synthesis or reduced protein syntheticefficiencies. The aim of the present study was to characterise tissue composition of nucleic acids and rates of protein synthesis in non-cardiac tissues, such as liver, skeletal muscle (i.e., the Type I fibre-predominant soleus and Type II fibre-predominant plantaris), kidney, bone (tibia), skin and the gastrointestinal tract in a genetic model of hypertension (i.e., spontaneously hypertensive rats (SHRs), 15 weeks old) compared to their geneticaged-matched counterparts, i.e., normotensive Wistar-Kyoto (WKY) controls. Rates of protein synthesis were measured in vivo after injection with a flooding dose of L-[4-H-3]phenylalanine. The results showed changed tissue wet weights (g per organ) for plantaris ( + 10%, P < 0.05), liver ( + 25%, P < 0.01), brain (-9%, P < 0.01), jejunum ( + 39%, P < 0.001) and tibia ( + 17%, P < 0.001) in SHRs compared to WKY controls. Protein content (g or mg per organ) was increased in the liver ( + 32%, P < 0.01) and tibia ( + 37%, P < 0.05). RNA contents (mg per organ) were increased in plantaris ( + 17%,P < 0.01), liver ( + 22%, P < 0.01) and jejunum (+ 11%, P < 0.05). DNA (mgper organ) was increased in plantaris (+ 16%, P < 0.025) and jejunum (+ 12%, P < 0.025). The protein synthetic capacities (i.e., C-s, mg RNA/g protein) were higher in soleus ( + 41%, P < 0.01) and plantaris (+ 6%, P < 0.05) muscles of SHRs compared to WKYs, whereas values were lower in liver (- 11%, P < 0.01) and kidney (-6%, P < 0.01) of SHRs compared to WKYs. The fractional rate of protein synthesis (i.e., k(s), the percentage of the protein pool renewed each day) was not significantly different for any of the tissues, though the rate of protein synthesis per unit RNA (i.e., k(RNA), mg protein/day per mg RNA) was reduced in the soleus (-24%, P < 0.05) and the synthesis rate per unit DNA, i.e., k(DNA) (mg protein/day per mg DNA) was increased in the tibia ( + 31%, P < 0.025). This is the first report of significant differences between indices of protein metabolism in extracardiac tissues in hypertension, which may reflect endocrine factors and/or the systemicinfluence of hypertension per se. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:02:53