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Titolo:
E1A is sufficient by itself to induce apoptosis independent of p53 and other adenoviral gene products
Autore:
Putzer, BM; Stiewe, T; Parssanedjad, K; Rega, S; Esche, H;
Indirizzi:
Univ Essen Gesamthsch, Sch Med, Inst Mol Biol Canc Res, D-45122 Essen, Germany Univ Essen Gesamthsch Essen Germany D-45122 Res, D-45122 Essen, Germany
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 2, volume: 7, anno: 2000,
pagine: 177 - 188
SICI:
1350-9047(200002)7:2<177:EISBIT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOBLASTOMA PROTEIN; P53-INDEPENDENT APOPTOSIS; HISTONE DEACETYLASE; TUMOR-SUPPRESSOR; 19-KILODALTON PROTEIN; EARLY REGION-4; DNA-SYNTHESIS; IN-VIVO; BINDING; CELLS;
Keywords:
E1A; apoptosis; RB; p300; transformation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Putzer, BM Univ Essen Gesamthsch, Sch Med, Inst Mol Biol Canc Res, Hufelandstr 55, D-45122 Essen, Germany Univ Essen Gesamthsch Hufelandstr 55 EssenGermany D-45122 any
Citazione:
B.M. Putzer et al., "E1A is sufficient by itself to induce apoptosis independent of p53 and other adenoviral gene products", CELL DEAT D, 7(2), 2000, pp. 177-188

Abstract

Induction of apoptosis seems to be a key function in maintaining normal cell growth by exerting negative controls on cell proliferation and suppressing tumorigenesis, The adenovirus E1A oncogene shows both cell cycle progression and apoptotic functions. To understand the mechanism of El A-induced apoptosis, the apoptotic function of E1A 13S was investigated in p53-null cells. We show here that E1A is sufficient by itself to induce substantial apoptosis independent of p53 and other adenoviral genes. The apoptotic function of E1A is accompanied by processing of caspase-3 and cleavage of poly(ADP-ribose)-polymerase. Cell death is significantly blocked by the caspase inhibitor zVAD-fmk and when coexpressed with E1B19K, Bcl-2 or the retinoblastoma protein (RB), Analyses of E1A mutants indicated that the apoptotic activity of E1A correlates closely with the ability to bind the key regulators of E2F1-induced apoptosis, p300 and RB. Finally, in vivo relevance of down-modulation of p53-independent apoptosis for efficient transformation is demonstrated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:21:20