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Titolo:
MAP2 and neurogranin as markers for dendritic lesions in CNS injury. An immunohistochemical study in the rat
Autore:
Li, GL; Farooque, M; Lewen, A; Lennmyr, F; Holtz, A; Olsson, Y;
Indirizzi:
Univ Uppsala Hosp, Neuropathol Lab, Dept Genet & Pathol, Res Grp Neuropathol, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 pathol, S-75185 Uppsala, Sweden Univ Uppsala Hosp, Dept Neurosci, Neurosurg Unit, Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden rosci, Neurosurg Unit, Uppsala, Sweden Univ Uppsala Hosp, Dept Med, Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden ppsala Hosp, Dept Med, Uppsala, Sweden
Titolo Testata:
APMIS
fascicolo: 2, volume: 108, anno: 2000,
pagine: 98 - 106
SICI:
0903-4641(200002)108:2<98:MANAMF>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROTUBULE-ASSOCIATED PROTEIN-2; MIDDLE CEREBRAL-ARTERY; TRAUMATIC BRAIN INJURY; KINASE-C SUBSTRATE; SPINAL-CORD; ISCHEMIC DEGRADATION; SENSITIVE MARKER; LOCALIZATION; PHOSPHORYLATION; RC3;
Keywords:
dendrite; injury; MAP2; neurogranin; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Li, GL Univ Uppsala Hosp, Neuropathol Lab, Dept Genet & Pathol, Res Grp Neuropathol, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 S-75185 Uppsala, Sweden
Citazione:
G.L. Li et al., "MAP2 and neurogranin as markers for dendritic lesions in CNS injury. An immunohistochemical study in the rat", APMIS, 108(2), 2000, pp. 98-106

Abstract

We compared two staining methods for the demonstration of dendrites under normal and pathological conditions of the rat central nervous system. MAP2-and neurogranin immunohistochemistry was applied to samples from normal tissue, spinal cord subjected to graded compression trauma, cerebral cortex following contusion trauma, and brains with focal ischemic lesions induced by occlusion of the middle cerebral artery (MCAO). Normal rats showed MAP2 immunoreactivity in nerve cell bodies and dendrites of brain and spinal cord. However, neurogranin staining was present only in nerve cell bodies and dendrites of the normal brain, and not in the spinal cord. Reduction of MAP2 immunoreactivity was seen in lesions of spinal cords subjected to compression trauma. Neurogranin staining was of no value in this experimental condition since it was not present under normal conditions. The brain contusions showed loss of both MAP2- and neurogranin immunoreactivity at the site of the lesion. MCAO resulted in an extensive loss of MAP2- and neurogranin staining in the ipsilateral hemisphere. In conclusion, our study shows that MAP2 immunostaining is a sensitive method for identifying dendritic lesions of various CNS injuries in the rat. Neurogranin immunostaining is an alternative method for investigations of dendritic pathology in the brain but not in the spinal cord.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 09:18:16