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Titolo:
Coumarin production in Mikania glomerata callus
Autore:
Pereira, AMS; Franca, SC; Camara, FLA;
Indirizzi:
Univ Ribeirao Preto, UNAERP, Dept Biotecnol Vegetal, BR-14096380 Ribeirao Preto, SP, Brazil Univ Ribeirao Preto Ribeirao Preto SP Brazil BR-14096380BCto, SP, Brazil
Titolo Testata:
WOCMAP-2: SECOND WORLD CONGRESS ON MEDICINAL AND AROMATIC PLANTS FOR HUMANWELFARE, PROCEEDINGS
fascicolo: 502, , anno: 1999,
pagine: 357 - 361
SICI:
0567-7572(1999):502<357:CPIMGC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SUSPENSION CULTURES; GROWTH; ACID;
Keywords:
guaco; Mikania glomerata; tissue culture; medicinal plant;
Tipo documento:
Article
Natura:
Collana
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Pereira, AMS Univ Ribeirao Preto, UNAERP, Dept Biotecnol Vegetal, BR-14096380 Ribeirao Preto, SP, Brazil Univ Ribeirao Preto Ribeirao Preto SP Brazil BR-14096380 BCl
Citazione:
A.M.S. Pereira et al., "Coumarin production in Mikania glomerata callus", ACTA HORT, (502), 1999, pp. 357-361

Abstract

Cell cultures of Mikania glomerata Sprengel were established with leaf segments cultured on White medium supplemented with 1 mg/L BA and 3 mg/L NAA. Different types and concentrations of growth regulators were tested for callus maintenance. Determination of coumarin content was performed in HPLC using authentic coumarin standard. Growth regulator concentration affected biomass and coumarin accumulation. Cultures developed in semisolid medium containing both BA and NAA exhibited enhanced biomass production as well as coumarin accumulation. In the most favorable conditions tested, cells accumulated 25 mu g/g of dry weight what is much inferior to the yield already reported in intact plants (5 mg/g of dry weight). However, results obtained sofar suggest several alternatives for culture manipulation in order to optimize the productivity of coumarin by M. glomerata cultured cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:23:12