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Titolo:
Differential calcium transport disturbances in renal membrane vesicles after cadmium-metallothionein injection in rats
Autore:
Leffler, PE; Jin, TY; Nordberg, GF;
Indirizzi:
Umea Univ, Dept Occupat & Environm Med, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 cupat & Environm Med, S-90187 Umea, Sweden
Titolo Testata:
TOXICOLOGY
fascicolo: 3, volume: 143, anno: 2000,
pagine: 227 - 234
SICI:
0300-483X(20000307)143:3<227:DCTDIR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
KIDNEY CORTEX; NEPHROTOXICITY; NEPHROPATHY; TOXICITY;
Keywords:
nephrotoxicity; renal membrane vesicles; cadmium-metallothionein; calcium transport; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Leffler, PE Umea Univ, Dept Occupat & Environm Med, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 ronm Med, S-90187 Umea, Sweden
Citazione:
P.E. Leffler et al., "Differential calcium transport disturbances in renal membrane vesicles after cadmium-metallothionein injection in rats", TOXICOLOGY, 143(3), 2000, pp. 227-234

Abstract

Cadmium-metallothionein (CdMT) induced calciuria may result from disturbedcalcium (Ca) transport through the renal tubular epithelium. The present study aimed at defining time of onset and the degree of disturbed calcium transport. Kidneys were obtained from rats at 4, 12 and 24 h after a single injection of CdMT (dose 0.4 mg Cd/kg b.w.), and compared to saline injected controls. Rapid-filtration Ca-45-assays were performed on basolateral and luminal membrane vesicles, isolated from kidney cortex using a sequential ultracentrifugation procedure. Luminal Ca-45 uptake was increased at 4 h and then declined to about 80% of controls, suggesting an early phase perturbation of Ca absorption. Basolateral Ca-45 uptake was reduced to less than 50%of controls, starting already at 4 h while Ca-45 binding was reduced at 8 h. This may reflect an inhibited basolateral Ca pump mechanism after the binding step. Since the Ca pump normally expels Ca from the cell, an accumulation of intracellular calcium was indicated. Metal analysis verified a four-fold increase of Ca in kidney cortex at 24 h. This suggests that Cd impacton tubular cells involves disturbances on cellular absorption as well as expulsion of Ca. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:53:30