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Titolo:
Role of climbing fibers in determining the spatial patterns of activation in the cerebellar cortex to peripheral stimulation: An optical imaging study
Autore:
Hanson, CL; Chen, G; Ebner, TJ;
Indirizzi:
Univ Minnesota, Dept Neurosci, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 urosci, Minneapolis, MN 55455 USA Univ Minnesota, Dept Neurosurg, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 rosurg, Minneapolis, MN 55455 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 96, anno: 2000,
pagine: 317 - 331
SICI:
0306-4522(2000)96:2<317:ROCFID>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFERIOR OLIVARY NEURONS; PURKINJE-CELL COMPLEX; AGGLUTININ-HORSERADISH-PEROXIDASE; CENTRAL-NERVOUS-SYSTEM; SPIKE ACTIVITY; GRANULE CELL; SPINOCEREBELLAR PROJECTIONS; FUNCTIONAL-ORGANIZATION; OSCILLATORY PROPERTIES; ANTEROGRADE TRANSPORT;
Keywords:
Neutral Red; cerebellum; climbing fibers; inferior olive; activation map; optical imaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
89
Recensione:
Indirizzi per estratti:
Indirizzo: Ebner, TJ Univ Minnesota, Dept Neurosci, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 nneapolis, MN 55455 USA
Citazione:
C.L. Hanson et al., "Role of climbing fibers in determining the spatial patterns of activation in the cerebellar cortex to peripheral stimulation: An optical imaging study", NEUROSCIENC, 96(2), 2000, pp. 317-331

Abstract

The spatial patterns of activation in the rat cerebellar cortex evoked by ipsilateral face stimulation were mapped using optical imaging based on thepH sensitive dye, Neutral Red. The aims of the study were to characterize the optical responses evoked by peripheral stimulation and test the hypothesis that the resultant parasagittal banding is due to climbing fiber activation. In the anesthetized rat Crus I and II of the cerebellar cortex were stained with Neutral Red. Epi-fluorescent changes produced by a train of stimuli (5-10 s and 4-20 Hz) to the ipsilateral face were monitored in time using a fast, high resolution charge-coupled device camera. The patterns of activation were quantified using a two-dimensional fast Fourier transform analysis that removed signals with high spatial frequencies and minimized thecontribution of horizontal structural elements (i.e. blood vessels). The dominant spatial pattern of activation evoked by face stimulation was that of parasagittal bands. The bands were highly frequency-dependent and were elicited most strongly by stimulus frequencies in the range of 6-8 Hz. There was a large fall-off in the response for frequencies above and below. The optical signal evoked by face stimulation built up over a period of 10 s andthen gradually decayed, Within a folium the individual parasagittal bands exhibited some frequency and temporal specificity. Stimulation of the contralateral inferior olive also resulted in the activation of parasagittal bands with characteristics similar to the bands evoked by face stimulation, including a preferred stimulus frequency which peaked at 10 Hz. Injection of lidocaine into the contralateral inferior olive blocked the parasagittal bands evoked by ipsilateral face stimulation, while control injections of saline had no effect. The results confirm that a parasagittal banding pattern is a dominant feature of the functional architecture of the cerebellar cortex. The parasagittal banding pattern observed with Neutral Red is due primarily to the activation of climbing fiber afferents. The frequency tuning of the responses, with the preference for peripheral stimuli of 6-8 Hz, is in agreement with previous findings that the inferior olive is inherently rhythmic. These observations support the hypothesis that inferior olivary neurons are dynamically coupled into groups that activate parasagittal bands of Purkinje cells inthe cerebellar cortex. The frequency tuning also supports the hypothesis that the climbing fiber system is involved with timing. Activation of this afferent system may require stimuli with appropriate frequency content and stimuli synchronized to the rhythmicity of the inferior olive. (C) 2000 IBRO. Published by Elsevier Science Ltd.

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Documento generato il 01/12/20 alle ore 22:51:16