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Titolo:
Involvement of progesterone in gonadotrophin-induced pituitary adenylate cyclase-activating polypeptide gene expression in preovulatory follicles of rat ovary
Autore:
Park, JI; Kim, WJ; Wang, L; Park, HJ; Lee, J; Park, JH; Kwon, HB; Tsafriri, A; Chun, SY;
Indirizzi:
Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Dept Biol, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Weizmann Inst Sci, Bernhard Zondek Hormone Res Lab, Dept Regulat Biol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 Biol, IL-76100 Rehovot, Israel
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 3, volume: 6, anno: 2000,
pagine: 238 - 245
SICI:
1360-9947(200003)6:3<238:IOPIGP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMED IMMATURE RAT; GRANULOSA-CELLS; RECEPTOR GENE; CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE; REGULATORY ELEMENTS; OVULATION; BINDING; CLONING; PACAP; IDENTIFICATION;
Keywords:
gonadotrophin; ovary; ovulation; Pacap; progesterone receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Chun, SY Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 00757, South Korea
Citazione:
J.I. Park et al., "Involvement of progesterone in gonadotrophin-induced pituitary adenylate cyclase-activating polypeptide gene expression in preovulatory follicles of rat ovary", MOL HUM REP, 6(3), 2000, pp. 238-245

Abstract

The present study was designed to determine whether progesterone might have a role in gonadotrophin-induced pituitary adenylate cyclase-activating polypeptide (Pacap) gene expression in rat ovary. Northern blot analysis revealed that treatment of pregnant mare's serum gonadotrophin (PMSG)-primed immature rats with the progestin antagonist RU486 or an inhibitor of 3 beta-hydroxysteroid dehydrogenase epostane, 1 h before HCG, resulted in a dose-dependent inhibition of the HCG-induced Pacap gene expression. In-situ hybridization demonstrated that the number of pre-ovulatory follicles expressing Pacap mRNA in their granulosa cells was greatly reduced in ovaries treated with RU486. Moreover, the suppressive effect of RU486 or epostane on the LH-induced Pacap gene expression in cultured pre-ovulatory follicles was reversed by cotreatment with the synthetic progestin R5020. We further cloned the 5'-flanking region of the rat Pacap gene and identified the presence of a consensus progesterone receptor element. When luciferase fusion genes containing Pacap gene promoter were transiently transfected into granulosa cells of pre-ovulatory follicles, luciferase activity was markedly stimulated by LH. Treatment with RU486 or epostane resulted in partial suppression of LH-stimulated PACAP promoter activity. Taken together, these results indicate that progesterone, acting through progesterone receptors, plays a role in gonadotrophin induction of Pacap gene expression in granulosa cells of pre-ovulatory follicles, and thereby may be involved in the process of ovulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:09:02