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Titolo:
Activation of the MN/CA9 gene is associated with hypomethylation carcinomacell lines
Autore:
Cho, M; Grabmaier, K; Kitahori, Y; Hiasa, Y; Nakagawa, Y; Uemura, H; Hirao, Y; Ohnishi, T; Yoshikawa, K; Ooesterwijk, E;
Indirizzi:
Nara Med Univ, Dept Urol, Kashihara, Nara 6348521, Japan Nara Med Univ Kashihara Nara Japan 6348521 Kashihara, Nara 6348521, Japan Nara Med Univ, Dept Pathol 2, Kashihara, Nara 6348521, Japan Nara Med Univ Kashihara Nara Japan 6348521 Kashihara, Nara 6348521, Japan Nara Med Univ, Dept Biol, Kashihara, Nara 6348521, Japan Nara Med Univ Kashihara Nara Japan 6348521 Kashihara, Nara 6348521, Japan Aichi Med Univ, Dept Pathol 2, Aichi, Japan Aichi Med Univ Aichi JapanAichi Med Univ, Dept Pathol 2, Aichi, Japan Univ Nijmegen Hosp, Dept Urol, NL-6500 HB Nijmegen, Netherlands Univ Nijmegen Hosp Nijmegen Netherlands NL-6500 HB Nijmegen, Netherlands
Titolo Testata:
MOLECULAR CARCINOGENESIS
fascicolo: 3, volume: 27, anno: 2000,
pagine: 184 - 189
SICI:
0899-1987(200003)27:3<184:AOTMGI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBONIC-ANHYDRASE; DNA METHYLATION; NORMAL KIDNEY; EXPRESSION; PROTEIN; MN; CARCINOGENESIS; BIOMARKER; LEUKEMIA; IX;
Keywords:
MN/CA9; hypomethylation; renal cell carcinoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Cho, M Nara Med Univ, Dept Urol, Kashihara, Nara 6348521, Japan Nara Med Univ Kashihara Nara Japan 6348521 ra, Nara 6348521, Japan
Citazione:
M. Cho et al., "Activation of the MN/CA9 gene is associated with hypomethylation carcinomacell lines", MOL CARCINO, 27(3), 2000, pp. 184-189

Abstract

The MN/CA9 (G250) gene expressed in the normal alimentary tract in a tissue-specific manner is often activated in renal cell carcinomas. To cast light on the activation mechanism, we examined the methylation status of this gene in seven human renal cell carcinoma cell lines (SKRC-01, -06, -10, -12,-14, -44, and -59) and three normal kidney tissue samples by using the bisulfite genomic sequencing protocol. CpG methylation was measured at seven locations in the MN/CA9 5' region. MN/CA9 transcripts were detected by reverse transcription-polymerase chain reaction in five of the renal cell carcinoma cell lines (SKRC-01, -06, -10, -44, and -59). These MN/CA9 positive cell lines showed hypomethylation, whereas the remaining two cell lines (SKRC-12, and -14), and three normal kidney tissue samples without transcripts demonstrated hypermethylation. Treatment with the demethylating agent 5-aza-2'-deoxycytidine resulted in activation of the MN/CA9 gene in the negative cell lines (SKRC-12 and -14). These data suggest that hypomethylation in the5' region may have a major role in expression of the MN/CA9 gene in renal cell carcinoma cells. Mol. Carcinog. 27:184-189, 2000. (C) 2000 Wiley-Liss,Inc.

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Documento generato il 05/04/20 alle ore 03:01:14