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Titolo:
Green fluorescent protein gene insertion of Sendai virus infection in nudemice: Possibility as an infection tracer
Autore:
Agungpriyono, DR; Yamaguchi, R; Uchida, K; Tohya, Y; Kato, A; Nagai, Y; Asakawa, M; Tateyama, S;
Indirizzi:
Miyazaki Univ, Fac Agr, Dept Vet Pathol, Miyazaki 8892155, Japan Miyazaki Univ Miyazaki Japan 8892155 Vet Pathol, Miyazaki 8892155, Japan Kagoshima Univ, Fac Agr, Dept Vet Microbiol, Kagoshima 8900065, Japan Kagoshima Univ Kagoshima Japan 8900065 crobiol, Kagoshima 8900065, Japan Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Tokyo 2080011, Japan Natl Inst Infect Dis Tokyo Japan 2080011 e Control, Tokyo 2080011, Japan Natl Inst Infect Dis, AIDS Res Ctr, Shinjuku Ku, Tokyo 1628640, Japan NatlInst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan DNAVEC Res Inst, Tsukuba, Ibaraki 3050856, Japan DNAVEC Res Inst Tsukuba Ibaraki Japan 3050856 uba, Ibaraki 3050856, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 2, volume: 62, anno: 2000,
pagine: 223 - 228
SICI:
0916-7250(200002)62:2<223:GFPGIO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO MARKER; PNEUMONIA; PATHOGENESIS; REPLICATION; EXPRESSION; RESISTANT; RECOVERY; STRAINS;
Keywords:
BALB/c nude (nu/nu) mouse; green fluorescent protein; recombinant Sendai virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaguchi, R Miyazaki Univ, Fac Agr, Dept Vet Pathol, Nishi 1-1,Gakuen Kibana Dai, Miyazaki 8892155, Japan Miyazaki Univ Nishi 1-1,Gakuen Kibana Dai Miyazaki Japan 8892155
Citazione:
D.R. Agungpriyono et al., "Green fluorescent protein gene insertion of Sendai virus infection in nudemice: Possibility as an infection tracer", J VET MED S, 62(2), 2000, pp. 223-228

Abstract

The green fluorescent protein (GFP) marker from jellyfish Aequorea victoria is considered to have potential use in the study of host-pathogen relationships, by tracing infections in living cells, organs and animals. We compared the pathogenicity of Sendai virus with an inserted GFP gene (GFP-SeV with that of its wild-type (Wt-SeV) to determine the usefulness of the recombinant virus in long-term infection of BALB/c nude (nu/nu) mice. The resultsindicated that the presence of GFP in infected cells could be analyzed easily and sensitively. GFP helped in identifying and in understanding the cellular sites of viral replication in vitro and in vivo. However, the GFP insertion into the Wt-SeV genome, led to decreased pathogenicity, altering thein vivo viral kinetics.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 22:13:51