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Titolo:
Mass spectral investigations on microorganisms
Autore:
Krishnamurthy, T; Rajamani, U; Ross, PL; Jabhour, R; Nair, H; Eng, J; Yates, J; Davis, MT; Stahl, DC; Lee, TD;
Indirizzi:
USA, Edgewood CB Ctr, R&T Technol, Aberdeen Proving Ground, MD 21010 USA USA Aberdeen Proving Ground MD USA 21010 een Proving Ground, MD 21010 USA Geoctr, Gunpowder Branch, Aberdeen Proving Ground, MD 21010 USA Geoctr Aberdeen Proving Ground MD USA 21010 Proving Ground, MD 21010 USA Univ Washington, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 iv Washington, Seattle, WA 98195 USA City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA 91010 USA City Hope Natl Med Ctr Duarte CA USA 91010 Res Inst, Duarte, CA 91010 USA
Titolo Testata:
JOURNAL OF TOXICOLOGY-TOXIN REVIEWS
fascicolo: 1, volume: 19, anno: 2000,
pagine: 95 - 117
SICI:
0731-3837(2000)19:1<95:MSIOM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASSISTED-LASER-DESORPTION/IONIZATION; CHAIN-REACTION PRODUCTS; RAPID IDENTIFICATION; DNA-POLYMERASE; WHOLE CELLS; SPECTROMETRY; AMPLIFICATION; BACTERIA; CHROMATOGRAPHY; PATTERNS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Krishnamurthy, T USA, Edgewood CB Ctr, R&T Technol, Aberdeen Proving Ground, MD 21010 USA USA Aberdeen Proving Ground MD USA 21010 d, MD 21010 USA
Citazione:
T. Krishnamurthy et al., "Mass spectral investigations on microorganisms", J TOX-TOX R, 19(1), 2000, pp. 95-117

Abstract

Bacterial cells undergo lysis readily, when suspended in mild aqueous acids, and release the cellular proteins along with other biomolecules. Molecular masses of the protein biomarkers released in-situ from individual intactbacterial cells could be directly measured by mass spectrometry. Limited sample clean up may be required at times, prior to mass spectral analysis, to remove any ionizable impurities such as salts, buffers and deergents. Themarker proteins specific for individual genus, species and strains were determined by the comparison of the biomarkers measured for several closely related organisms. Even though there is a probability of over 4000 cellular proteins expressed in any singlebacterial cell, only a small fraction of the projected marker proteins rue identified consistently during the process. This could be due to the variation in the ionization properties of the proteins ansi the limited energy available to prompt their ionization. Variation in the sample processing and culture conditions had little effect in themarker proteins observed during the process. This experimental procedure enables the distinction of gram positive as well as gram negative cellular pathogens and their corresponding nonpathogenic counterparts. The identity of few bacterial cells present in unknown samples can be easily, rapidly andaccurately established by adopting a procedure involving simple sample processing followed by direct mass spectral analysis and data prosessing. Thus, an uncomplicated approach has been developed to resolve a complex probleminvolving cellular pathogens. This method has enormous application potential in the rapid identification and subsequent prevention of any potential health hazard caused by the pathogenic bacteria, either under natural or induced conditions. There is a great potential for the total automation of theentire process in the future for simpler but more effective unattended operations in the laboratory as well as in the field.

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Documento generato il 28/09/20 alle ore 05:21:04