Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Liquid chromatographic assay of nifedipine in human plasma and its application to pharmacokinetic studies
Autore:
Abou-Auda, HS; Najjar, TA; Al-Khamis, KI; Al-Hadiya, BM; Ghilzai, NM; Al-Fawzan, NF;
Indirizzi:
King Saud Univ, Coll Pharm, Dept Clin Pharm, Riyadh 11451, Saudi Arabia King Saud Univ Riyadh Saudi Arabia 11451 arm, Riyadh 11451, Saudi Arabia
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 2, volume: 22, anno: 2000,
pagine: 241 - 249
SICI:
0731-7085(200003)22:2<241:LCAONI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY GAS-CHROMATOGRAPHY; CARDIOVASCULAR DISORDERS; METABOLITE; URINE; EXTRACTION; BLOOD;
Keywords:
high-performance liquid chromatography; nifedipine; photostability; freeze/thaw stability; pharmacokinetics; therapeutic drug monitoring;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Auda, HS King Saud Univ, Coll Pharm, Dept Clin Pharm, POB 2457, Riyadh 11451, SaudiArabia King Saud Univ POB 2457 Riyadh Saudi Arabia 11451 udiArabia
Citazione:
H.S. Abou-Auda et al., "Liquid chromatographic assay of nifedipine in human plasma and its application to pharmacokinetic studies", J PHARM B, 22(2), 2000, pp. 241-249

Abstract

A highly sensitive, selective and reproducible reversed-phase high-performance liquid chromatographic method has been developed for the determinationof nifedipine iii human plasma with minimum sample preparation. The methodis sensitive to 3 ng/ml in plasma, with acceptable within- and between-dayreproducibilities and linearity (r(2) > 0.99) over a concentration range from 10-200 ng/ml. Acidified plasma samples were extracted using diethyethercontaining diazepam as internal standard and chromatographic separation was accomplished on C-18 column using a mobile phase consisting of acetonitrile, methanol and water (35:17:48, v/v). The within-day precision ranged from 2.22 to 4.64% and accuracy ranged from 102.4-106.4%. The day-to-day precision ranged from 2.34-7.07% and accuracy from 95.1-100.1%. The relative recoveries of nifedipine from plasma ranged from 91.0-107.3% whereas extraction recoveries were 88.6-93.3%. Following eight 6-week freeze-thaw cycles, nifedipine in plasma samples proved to be stable with accuracy ranging from 0.64 to 3.0% and precision ranging from 3.6 to 4.15%. Nifedipine was also found to be photostable for at least 120 min in plasma, 30 min in blood and for 60 min in aqueous solutions after exposure to light. The method is sensitive and reliable for pharmacokinetic studies and therapeutic drug monitoring of nifedipine in humans after the oral administration of immediate-release capsules and sustained-release tablets to five healthy subjects. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:12:43