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Titolo:
CCAAT ENHANCER BINDING-PROTEIN-EPSILON IS PREFERENTIALLY UP-REGULATEDDURING GRANULOCYTIC DIFFERENTIATION AND ITS FUNCTIONAL VERSATILITY ISDETERMINED BY ALTERNATIVE USE OF PROMOTERS AND DIFFERENTIAL SPLICING/
Autore:
YAMANAKA R; KIM GD; RADOMSKA HS; LEKSTROMHIMES J; SMITH LT; ANTONSON P; TENEN DG; XANTHOPOULOS KG;
Indirizzi:
NATL HUMAN GENOME RES INST,CLIN GENE THERAPY BRANCH,NIH,BLDG 10,ROOM 10C103 BETHESDA MD 20892 NATL HUMAN GENOME RES INST,CLIN GENE THERAPY BRANCH,NIH BETHESDA MD 20892 HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,DEPT MED,HEMATOL ONCOL DIV, BOSTON MA 02215 ROYAL CANC HOSP,INST CANC RES,HADDOW LABS SUTTON SM2 5NG SURREY ENGLAND
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 12, volume: 94, anno: 1997,
pagine: 6462 - 6467
SICI:
0027-8424(1997)94:12<6462:CEBIPU>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
C/EBP FAMILY MEMBERS; NF-KAPPA-B; MESSENGER-RNA; TRANSCRIPTIONAL ACTIVATORS; DNA-BINDING; GENE; EXPRESSION; CELLS; NF-IL6; REGION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
R. Yamanaka et al., "CCAAT ENHANCER BINDING-PROTEIN-EPSILON IS PREFERENTIALLY UP-REGULATEDDURING GRANULOCYTIC DIFFERENTIATION AND ITS FUNCTIONAL VERSATILITY ISDETERMINED BY ALTERNATIVE USE OF PROMOTERS AND DIFFERENTIAL SPLICING/", Proceedings of the National Academy of Sciences of the United Statesof America, 94(12), 1997, pp. 6462-6467

Abstract

CCAAT/enhancer binding protein (C/EBP) epsilon is a recently cloned member of the C/EBP family of transcription factors and is expressed exclusively in cells of hematopoietic origin, The human C/EBP epsilon gene is transcribed by two alternative promoters, P alpha and P beta. A combination of differential splicing and alternative use of promoters generates four mRNA isoforms, of 2.6 kb and 1.3-1.5 kb in size, These transcripts can encode three proteins of calculated molecular mass 32.2 kDa, 27.8 kDa, and 14.3 kDa, Accordingly, Western blots with antibodies specific for the DNA-binding domain, that is common to all forms, identify multiple proteins, C/EBP epsilon mRNA was greatly induced during in vitro granulocytic differentiation of human primary CD34(+) cells, Retinoic acid treatment of HL60 promyelocytic leukemia cells for 24 hr induced C/EBP epsilon mRNA levels by 4-fold, while prolonged treatment gradually reduced mRNA expression to pretreatment levels. Transient transfection experiments with expression vectors for two of the isoforms demonstrated that the 32.2-kDa protein is an activator of transcription of granulocyte colony-stimulating factor receptor promoter, while the 14.3-kDa protein is not. Thus, C/EBP epsilon is regulated in a complex fashion and may play a role in the regulation of genes involved in myeloid differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 11:22:35