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Titolo:
Maturational changes in rabbit renal basolateral membrane vesicle osmotic water permeability
Autore:
Quigley, R; Gupta, N; Lisec, A; Baum, M;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Pediat, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Med Ctr, Dept Pediat, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 r, Dept Internal Med, Dallas, TX 75235 USA
Titolo Testata:
JOURNAL OF MEMBRANE BIOLOGY
fascicolo: 1, volume: 174, anno: 2000,
pagine: 53 - 58
SICI:
0022-2631(20000301)174:1<53:MCIRRB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROXIMAL CONVOLUTED TUBULE; BRUSH-BORDER; LOCALIZATION; TRANSPORT; CHANNELS; CORTEX; KIDNEY; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Quigley, R Univ Texas, SW Med Ctr, Dept Pediat, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 pt Pediat, Dallas, TX 75235 USA
Citazione:
R. Quigley et al., "Maturational changes in rabbit renal basolateral membrane vesicle osmotic water permeability", J MEMBR BIO, 174(1), 2000, pp. 53-58

Abstract

We have recently demonstrated that while the osmotic water permeability (P-f) of neonatal proximal tubules is higher than that of adult tubules, the P-f of brush-border membrane vesicles from neonatal rabbits is lower than that of adults. The present study examined developmental changes in the water transport characteristics of proximal tubule basolateral membranes by determining aquaporin 1 (AQP1) protein abundance and the P-f in neonatal (10-14 days old) and adult rabbit renal basolateral membrane vesicles (BLMV). At25 degrees C the P-f of neonatal BLMV was significantly lower than the adult BLMV at osmotic gradients ranging from 40 to 160 mOsm/kg water. The activation energies for osmotic water movement were identical in the neonatal and adult BLMV (8.65 +/- 0.47 vs. 8.86 +/- 1.35 kcal . deg(-1) . mol(-1)). Reflection coefficients for sodium chloride and sodium bicarbonate were identical in both the neonatal and adult BLMV and were not different from one. Mercury chloride (0.5 mM) reduced osmotic water movement by 31.3 +/- 5.5% in the adult BLMV, but by only 4.0 +/- 4.0% in neonatal vesicles (P < 0.01). Adult BLMV AQP1 abundance was higher than that in the neonate. These data demonstrate that neonatal BLMV have a lower P-f and AQP1 protein abundance than adults and that a significantly greater fraction of water traverses the basolateral membrane lipid bilayer and not water channels in neonates compared to adults. The lower P-f of the neonatal BLMV indicates that the basolateral membrane is not responsible for the higher transepithelial P-f in the neonatal proximal tubule.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:00:02