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Titolo:
Effect of glycoprotein IIb/IIIa inhibitors on CD62p expression, platelet aggregates, and microparticles in vitro
Autore:
Matzdorff, AC; Kuhnel, G; Kemkes-Matthes, B; Pralle, H; Voss, R; Fareed, J;
Indirizzi:
Univ Giessen, Ctr Internal Med, Dept Cardiol, Dept Hematol Oncol, D-35385 Giessen, Germany Univ Giessen Giessen Germany D-35385 tol Oncol, D-35385 Giessen, Germany Loyola Univ, Div Hemostasis, Maywood, IL 60153 USA Loyola Univ Maywood ILUSA 60153 v, Div Hemostasis, Maywood, IL 60153 USA
Titolo Testata:
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
fascicolo: 3, volume: 135, anno: 2000,
pagine: 247 - 255
SICI:
0022-2143(200003)135:3<247:EOGIIO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY; FLOW-CYTOMETRY; ANTITHROMBOTIC EFFICACY; FIBRINOGEN BINDING; INDUCED ACTIVATION; SHAPE CHANGE; WHOLE-BLOOD; RECEPTOR; ANTIPLATELET; ANTAGONIST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Matzdorff, AC Univ Giessen, Ctr Internal Med, Dept Cardiol, Dept Hematol Oncol, Klin Str36, D-35385 Giessen, Germany Univ Giessen Klin Str 36 Giessen Germany D-35385 n, Germany
Citazione:
A.C. Matzdorff et al., "Effect of glycoprotein IIb/IIIa inhibitors on CD62p expression, platelet aggregates, and microparticles in vitro", J LA CL MED, 135(3), 2000, pp. 247-255

Abstract

Flow cytometry can detect platelet activation (CD62p), aggregate formation, microparticle formation, and glycoprotein IIb/IIIa (GP IIb/IIIa) receptoroccupancy in one sample at the level of single particles. We studied the effect of GP IIb/IIIa inhibitors on platelet activation with flow cytometry in vitro. Citrated whole blood was incubated with increasing concentrationsof three different GP IIb/IIIa inhibitors (c7E3, DMP728, XJ757), then thrombin or adenosine diphosphate (ADP) was added, and after 1 minute the sample was fixed. Samples with thrombin but without c7E3 had a decrease in platelet count, from a mean of 260,000 platelets/mu L to 56,000 platelets/mu L, and aggregates increased. Samples with concentrations of c7E3 that resultedin 80% or more receptor blockade had no decrease in platelet count, and noaggregates were formed, but the number of CD62p-positive single platelets increased from 1200 to 7400 platelets/mu L. The two other inhibitors (DMP 725, XJ757) or ADP instead of thrombin gave similar results. Microparticle formation did not change with platelet activation in the presence of a GP IIb/IIIa inhibitor. With small inhibitor doses resulting in <80% receptor blockade, the number of aggregates did not change or was even higher than thatin samples without inhibitor. GP IIb/IIIa inhibitors do prevent aggregate formation but they do not prevent activation of platelets. With GP IIb/IIIainhibition, more activated single platelets remain in the blood. One may expect an increasing number of circulating, activated platelets with the useof GP IIb/IIIa inhibitors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:37:57