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Titolo:
A polymorphism in the mouse Crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay
Autore:
Hallensleben, W; Biro, L; Sauder, C; Hausmann, J; Asensio, VC; Campbell, IL; Staeheli, P;
Indirizzi:
Univ Freiburg, Inst Med Mikrobiol & Hyg, Abt Virol, D-79008 Freiburg, Germany Univ Freiburg Freiburg Germany D-79008 Virol, D-79008 Freiburg, Germany Scripps Clin & Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 col, La Jolla, CA 92037 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 234, anno: 2000,
pagine: 149 - 151
SICI:
0022-1759(20000203)234:1-2<149:APITMC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Keywords:
ribonuclease protection assay; CXC chemokine; Crg-2/IP-10 gene polymorphism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
1
Recensione:
Indirizzi per estratti:
Indirizzo: Staeheli, P Univ Freiburg, Inst Med Mikrobiol & Hyg, Abt Virol, Hermann Herder Str 11,D-79008 Freiburg, Germany Univ Freiburg Hermann Herder Str 11 Freiburg Germany D-79008
Citazione:
W. Hallensleben et al., "A polymorphism in the mouse Crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay", J IMMUNOL M, 234(1-2), 2000, pp. 149-151

Abstract

The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:24:46