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Titolo:
Measurement of T-helper cytokines secreted by cord blood mononuclear cellsin response to allergens
Autore:
Devereux, G; Hall, AM; Barker, RN;
Indirizzi:
Inst Med Sci, Dept Med & Therapeut, Aberdeen AB25 2ZD, Scotland Inst Med Sci Aberdeen Scotland AB25 2ZD eut, Aberdeen AB25 2ZD, Scotland Dept Environm & Occupat Med, Aberdeen AB25 2ZD, Scotland Dept Environm & Occupat Med Aberdeen Scotland AB25 2ZD B25 2ZD, Scotland
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 234, anno: 2000,
pagine: 13 - 22
SICI:
0022-1759(20000203)234:1-2<13:MOTCSB>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERFERON-GAMMA PRODUCTION; SIGNAL-TRANSDUCTION; INTERLEUKIN-4; NEWBORNS; RISK;
Keywords:
interferon-gamma; interleukin-4; T-helper subsets; human cord blood mononuclear cells; allergens; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Barker, RN Inst Med Sci, Dept Med & Therapeut, Aberdeen AB25 2ZD, ScotlandInst Med Sci Aberdeen Scotland AB25 2ZD en AB25 2ZD, Scotland
Citazione:
G. Devereux et al., "Measurement of T-helper cytokines secreted by cord blood mononuclear cellsin response to allergens", J IMMUNOL M, 234(1-2), 2000, pp. 13-22

Abstract

It has been proposed that in utero factors may predispose towards the development of childhood atopy. To test this hypothesis, it will be necessary to measure T-helper cell (Th) cytokines secreted by human cord blood mononuclear cells (CBMC) stimulated by allergens. However, to date, it has proven impossible to measure allergen-specific CBMC secretion of the key Th cytokine interleukin-4 (IL-4) using conventional sandwich ELISA techniques. We report for the first time the successful measurement of IL-4 secreted by CBMCstimulated by the allergens timothy grass pollen and house dust mite extract. The method is an adaptation of a novel cell-based ELISA (celELISA), which demonstrated an increased (up to 20-fold) sensitivity to detect IL-4. The method is simple, precise, is no more costly than a conventional ELISA, and can identify individuals in a general population whose CBMC exhibit different cytokine biases in response to allergens. The frequency distribution of IL-4 and interferon-gamma (IFN-gamma) CBMC responses to allergens in thegeneral population approximates to a log-normal distribution, which will permit the application of linear regression techniques in the identificationof in utero factors which influence Th bias. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 13:26:17