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Titolo:
EXPRESSION OF A GENE ENCODING A TRANSFER-RNA SYNTHETASE-LIKE PROTEIN IS ENHANCED IN TUMORIGENIC HUMAN MYELOID-LEUKEMIA CELLS AND IS CELL-CYCLE STAGE-DEPENDENT AND DIFFERENTIATION-DEPENDENT
Autore:
SEN S; ZHOU HY; RIPMASTER T; HITTELMAN WN; SCHIMMEL P; WHITE RA;
Indirizzi:
UNIV TEXAS,MD ANDERSON CANC CTR,DIV LAB MED,BOX 54,1515 HOLCOMBE BLVDHOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANC CTR,DEPT CLIN INVEST HOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANC CTR,DEPT BIOMATH HOUSTON TX 77030 CARNEGIE MELLON UNIV,DEPT BIOL SCI PITTSBURGH PA 15218 MIT,DEPT BIOL CAMBRIDGE MA 02139
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 12, volume: 94, anno: 1997,
pagine: 6164 - 6169
SICI:
0027-8424(1997)94:12<6164:EOAGEA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHENYLALANYL-TRANSFER-RNA; THERMUS-THERMOPHILUS; SEQUENCE TAGS; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
S. Sen et al., "EXPRESSION OF A GENE ENCODING A TRANSFER-RNA SYNTHETASE-LIKE PROTEIN IS ENHANCED IN TUMORIGENIC HUMAN MYELOID-LEUKEMIA CELLS AND IS CELL-CYCLE STAGE-DEPENDENT AND DIFFERENTIATION-DEPENDENT", Proceedings of the National Academy of Sciences of the United Statesof America, 94(12), 1997, pp. 6164-6169

Abstract

We cloned a tumorigenic phenotype-associated cDNA encoding a tRNA synthetase-like protein from an acute-phase human myeloid leukemia cell line. The cDNA was isolated by reiterative subtraction of cDNAs synthesized from tumor-generating parental leukemia cells versus those from anontumorigenic variant of the same cells, The selected cDNA encodes aprotein that is a close homolog of one subunit of prokaryote and yeast phenylalanyl-tRNA synthetase (PheRS). The expressed protein reacts specificially with polyclonal antibodies raised against mammalian phenylalanyl-tRNA synthetase. Expression of the gene (designated CML33) wasdirectly confirmed by Northern blot hybridization to be substantiallyenhanced in the tumorigenic cells compared with the nontumorigenic variant. In addition, expression of CML33 in myeloid leukemia cells was sensitive to the stage of the cell cycle and to induction of differentiation. Although the relationship between these observations and the tumorigenic state of the human myeloid leukemia cell line used in thesestudies is unknown, to our knowledge, this is the first demonstrationin mammalian cells of tumor-selective and cell cycle stage- and differentiation-dependent expression of a member of the tRNA synthetase gene family.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:23:36