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Titolo:
Effect of cationic liposomes on intracellular trafficking and efficacy of antisense oligonucleotides in mouse peritoneal macrophages
Autore:
Takagi, T; Hashiguchi, M; Hiramatsu, T; Yamashita, F; Takakura, Y; Hashida, M;
Indirizzi:
Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Drug Delivery Res, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 ivery Res, Sakyo Ku, Kyoto 6068501, Japan
Titolo Testata:
JOURNAL OF DRUG TARGETING
fascicolo: 5, volume: 7, anno: 2000,
pagine: 363 -
SICI:
1061-186X(2000)7:5<363:EOCLOI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; SMOOTH-MUSCLE CELLS; IN-VIVO; CELLULAR UPTAKE; EXPRESSION; LIPIDS; RAT; OLIGODEOXYNUCLEOTIDES; DENDRIMERS; COMPLEXES;
Keywords:
antisense oligonucleotides; cationic liposomes; confocal macroscopy; inducible nitric oxide synthase; macrophages; intracellular trafficking;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Hashida, M Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Drug Delivery Res, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Sakyo Ku, Kyoto 6068501, Japan
Citazione:
T. Takagi et al., "Effect of cationic liposomes on intracellular trafficking and efficacy of antisense oligonucleotides in mouse peritoneal macrophages", J DRUG TAR, 7(5), 2000, pp. 363

Abstract

We have investigated the intracellular fate and antisense effect of oligonucleotide/cationic liposome complexes using phosphorothioate oligonucleotides (S-Oligo) targeted to inducible nitric oxide synthase in mouse peritoneal macrophages. Confocal laser microscopic analysis revealed that, after application of fluorescein isothiocyanate (FITC)-labeled S-Oligo alone, the intracellular localization of fluorescence exhibited a punctate pattern in the cytoplasm, suggesting that the oligonucleotides were mainly confined to the endosomal and/or lysosomal compartments. In the case of complexation with Lipofectin and DMRIE-C liposomes, cellular uptake of FITC-S-Oligo was notgreatly enhanced and the fluorescence localization in the cells was similar to that of FITC-S-Oligo alone. LipofectAMINE slightly enhanced cellular uptake of FITC-S-Oligo: however, the intracellular localization profile of FITC-S-Oligo remained largely unchanged. The antisense effect was slightly enhanced by LipofectAMINE under only very limited experimental conditions. It was concluded that cationic liposomes are not a potential carrier for S-OLigo in peritoneal macrophages because of their inability to promote the release of S-Oligo from the endosomal compartments to the cytosol over a non-toxic concentration range.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 15:36:58