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Titolo:
Molecular map of the desmosomal plaque
Autore:
North, AJ; Bardsley, WG; Hyam, J; Bornslaeger, EA; Cordingley, HC; Trinnaman, B; Hatzfeld, M; Green, KJ; Magee, AI; Garrod, DR;
Indirizzi:
Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England Northwestern Univ, Sch Med, Dept Pathol, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 Dept Pathol, Chicago, IL 60611 USA Northwestern Univ, Sch Med, Dept Dermatol, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 pt Dermatol, Chicago, IL 60611 USA Northwestern Univ, Sch Med, RH Lurie Canc Ctr, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 ie Canc Ctr, Chicago, IL 60611 USA Natl Inst Med Res, Div Membrane Biol, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA e Biol, London NW7 1AA, England Univ Halle Wittenberg, Fac Med, Mol Biol Grp, D-06097 Halle, Germany Univ Halle Wittenberg Halle Germany D-06097 Grp, D-06097 Halle, Germany
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 23, volume: 112, anno: 1999,
pagine: 4325 - 4336
SICI:
0021-9533(199912)112:23<4325:MMOTDP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT ANCHORAGE; SEGMENT POLARITY GENE; ADHESION MOLECULES; MULTIGENE FAMILY; INTERCELLULAR-JUNCTIONS; STRATIFIED EPITHELIA; CYTOPLASMIC DOMAINS; PEMPHIGUS-VULGARIS; ADHERING JUNCTIONS; HUMAN EPIDERMIS;
Keywords:
desmosome; immunogold; ultrastructure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
81
Recensione:
Indirizzi per estratti:
Indirizzo: North, AJ Univ Manchester, Sch Biol Sci, 3-239 Stopford Bldg,Oxford Rd, Manchester M13 9PT, Lancs, England Univ Manchester 3-239 Stopford Bldg,OxfordRd Manchester Lancs England M13 9PT
Citazione:
A.J. North et al., "Molecular map of the desmosomal plaque", J CELL SCI, 112(23), 1999, pp. 4325-4336

Abstract

Recent biochemical and molecular approaches have begun to establish the protein interactions that lead to desmosome assembly, To determine whether these associations occur in native desmosomes we have performed ultrastructural localisation of specific domains of the major desmosomal components and have used the results to construct a molecular map of the desmosomal plaque. Antibodies directed against the amino- and carboxy-terminal domains of desmoplakin, plakoglobin and plakophilin 1, and against the carboxy-terminal domains of desmoglein 3, desmocollin 2a and desmocollin 2b, were used for immunogold labelling of ultrathin cryosections of bovine nasal epidermis, For each antibody, the mean distance of the gold particles, and thus the detected epitope, from the cytoplasmic surface of the plasma membrane was determined quantitatively, Results showed that: (i) plakophilin, although previously shown to bind intermediate filaments in vitro, is localised extremely close to the plasma membrane, rather than in the region where intermediate filaments are seen to insert into the desmosomal plaque; (ii) while the 'a'form of desmocollin overlaps with plakoglobin and desmoplakin, the shorter'b' form may be spatially separated from them; (iii) desmoglein 3 extends across the entire outer plaque, beyond both desmocollins; (iv) the amino terminus of desmoplakin lies within the outer dense plaque and the carboxy terminus some 40 nm distant in the zone of intermediate filament attachment, This is consistent with a parallel arrangement of desmoplakin in dimers or higher order aggregates and with the predicted length of desmoplakin II, indicating that desmoplakin I may be folded or coiled. Thus several predictions from previous work were borne out by this study, but in other cases our observations yielded unexpected results. These results have significant implications relating to molecular interactions in desmosomes and emphasise the importance of applying multiple and complementary approaches to biological investigations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:54:42