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Titolo:
Neurotrophic factors in the human cornea
Autore:
You, LT; Kruse, FE; Volcker, HE;
Indirizzi:
Univ Heidelberg, Dept Ophthalmol, Augenklin, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 3, volume: 41, anno: 2000,
pagine: 692 - 702
SICI:
0146-0404(200003)41:3<692:NFITHC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; NORMAL HUMAN KERATINOCYTES; RECEPTOR TYROSINE KINASE; TRANSFORMING GROWTH-FACTOR-BETA-1; PC12 CELLS; SIGNAL-TRANSDUCTION; PROTEIN-KINASES; OCULAR SURFACE; LACRIMAL GLAND; CLONAL GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Kruse, FE Univ Heidelberg, Dept Ophthalmol, Augenklin, INF 400, D-69120 Heidelberg, Germany Univ Heidelberg INF 400 Heidelberg Germany D-69120 erg, Germany
Citazione:
L.T. You et al., "Neurotrophic factors in the human cornea", INV OPHTH V, 41(3), 2000, pp. 692-702

Abstract

PURPOSE. To investigate neurotrophic growth factors and corresponding receptors in human and rabbit corneal epithelium and stroma. METHODS. Transcription of nerve growth factor (NGF), neurotrophin 3 (NT-3), NT-4, brain-derived neurotrophic factor (BDNF), glial cell line- derived neurotrophic factor (GDNF), and receptors Trk A-E, was investigated by reverse transcription-polymerase chain reaction. DNA dot blot analysis allowed to estimate transcription levels. Single cell proliferation assays were performed using recombinant NGF, BDNF, and GDNF. Mitogen-activated protein kinase signal transduction was investigated with Western blot analysis using antibodies against-activated and total extracellular signal-regulated kinase(ERK) 1/2 and the jun N-terminal protein kinase (JNK) 1/2. RESULTS. Transcription of NGF, NT-3, BDNF, and Trk A, Trk B, Trk C, and Trk E receptors was detected in both ex vivo and cultured epithelium and stroma. Transcription of NT-4 was only detected in epithelium and transcriptionof GDNF only in stroma. Levels of transcription were higher for NT-3, NT-4, and the Trk receptors and lower for NGF, BDNF, and GDNF. NGF and GDNF stimulated both epithelial colony formation and proliferation, whereas BDNF only enhanced colony formation. Stromal proliferation was enhanced in serum-free medium. In epithelium, predominantly ERK 1 was activated by NGF, GDNF, and BDNF. In stromal cells NGF and GDNF stimulated phosphorylation of ERK 1and JNK 1. CONCLUSIONS. Neurotrophic factors and tyrosine kinase receptors are transcribed in the human cornea. GDNF and NGF stimulate corneal epithelial proliferation, and the effect of the latter might be mediated by activation of ERK 1. Neurotrophic factors have very specific effects on phosphorylation of ERK and JNK in epithelial and stromal cells. The differential expression ofNT-4 and GDNF suggests a regulatory function within the cytokine network of the cornea.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 04:19:54