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Titolo:
Inflammatory mediators induce sequestration of VE-cadherin in cultured human endothelial cells
Autore:
Alexander, JS; Alexander, BC; Eppihimer, LA; Goodyear, N; Haque, R; Davis, CP; Kalogeris, TJ; Carden, CL; Zhu, YN; Kevil, CG;
Indirizzi:
Louisiana State Univ, Med Ctr, Shreveport, LA 71130 USA Louisiana State Univ Shreveport LA USA 71130 tr, Shreveport, LA 71130 USA
Titolo Testata:
INFLAMMATION
fascicolo: 2, volume: 24, anno: 2000,
pagine: 99 - 113
SICI:
0360-3997(200004)24:2<99:IMISOV>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY LIPOPROTEIN; PROTEIN-KINASE-C; TIGHT JUNCTIONS; VASCULAR ENDOTHELIUM; ADHERENS JUNCTIONS; MEMBRANE-PROTEIN; PERMEABILITY; ENDOCYTOSIS; BARRIER; OCCLUDIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Alexander, JS Louisiana State Univ, Med Ctr, 1501 Kings Highway, Shreveport, LA 71130 USA Louisiana State Univ 1501 Kings Highway Shreveport LA USA 71130
Citazione:
J.S. Alexander et al., "Inflammatory mediators induce sequestration of VE-cadherin in cultured human endothelial cells", INFLAMMATIO, 24(2), 2000, pp. 99-113

Abstract

The mechanisms through which inflammatory mediators modify endothelial junctional structure are not well understood. Endothelial cells exposed to 1 mM H2O2, 0.1 mM histamine or 4 mM EDTA displayed decreased amounts of VE-cadherin on the cell surface in a time-dependent manner. H2O2 and EDTA-treatedcells showed a sustained reduction in surface VE-cadherin, but histamine (0.1 mM) decreased cell surface VE-cadherin only at 5 and 15 min, not at 30 and 60 min. Sequestering of VE-cadherin could also be visualized as a decrease in immunofluorescent labeling of endothelial junctions in fixed, non-extracted monolayers. However, junctional staining was observed in these cells after membrane extraction. This decreased surface expression of VE-cadherin was actin-filament, but not PKC/MAP kinase dependent. VE-cadherin binding to the cytoskeleton was decreased by EDTA, but was not diminished by histamine or H2O2. Therefore, by promoting sequestration of junctional cadherins, inflammatory mediators may decrease adhesive bonds between apposed endothelial cells and increase solute permeability.

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Documento generato il 26/09/20 alle ore 08:44:17