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Titolo:
Inhibition of immunoglobulin E response to Japanese cedar pollen allergen (Cry j 1) in mice by DNA immunization: different outcomes dependent on the plasmid DNA inoculation method
Autore:
Toda, M; Sato, H; Takebe, Y; Taniguchi, T; Saito, S; Inouye, S; Takemori, T; Sakaguchi, M;
Indirizzi:
Natl Inst Infect Dis, Dept Immunol, Shinjyuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 njyuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis, AIDS Res Ctr, Tokyo, Japan Natl Inst Infect Dis Tokyo Japan Infect Dis, AIDS Res Ctr, Tokyo, Japan Natl Inst Infect Dis, Infect Dis Surveillance Ctr, Tokyo, Japan Natl Inst Infect Dis Tokyo Japan ect Dis Surveillance Ctr, Tokyo, Japan Hayashibara Biochem Labs Inc, Okayama, Japan Hayashibara Biochem Labs IncOkayama Japan hem Labs Inc, Okayama, Japan Jikei Univ, Sch Med, Inst DNA Med, Div Mol Immunol, Tokyo, Japan Jikei Univ Tokyo Japan Med, Inst DNA Med, Div Mol Immunol, Tokyo, Japan
Titolo Testata:
IMMUNOLOGY
fascicolo: 2, volume: 99, anno: 2000,
pagine: 179 - 186
SICI:
0019-2805(200002)99:2<179:IOIERT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYPTOMERIA-JAPONICA POLLEN; SUGI BASIC-PROTEIN; J-I; MAJOR ALLERGEN; MONOCLONAL-ANTIBODIES; ANTIGENIC ANALYSES; GENE VACCINATION; INTERFERON-GAMMA; DENDRITIC CELLS; T-LYMPHOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Sakaguchi, M Natl Inst Infect Dis, Dept Immunol, Shinjyuku Ku, Toyama 1-23-1, Tokyo 1628640, Japan Natl Inst Infect Dis Toyama 1-23-1 Tokyo Japan 1628640 Japan
Citazione:
M. Toda et al., "Inhibition of immunoglobulin E response to Japanese cedar pollen allergen (Cry j 1) in mice by DNA immunization: different outcomes dependent on the plasmid DNA inoculation method", IMMUNOLOGY, 99(2), 2000, pp. 179-186

Abstract

To develop a new immunotherapy for Japanese cedar (Cryptomeria japonica; CJ) pollinosis, we evaluated the use of DNA immunization by inoculating micewith plasmid DNA encoding Cry j 1 as a CJ pollen major allergen (pCACJ1). Repeated intramuscular (i.m.) inoculation of BALB/c mice with pCACJ1 produced anti-Cry j 1 antibody responses, which were predominately of the immunoglobulin G2a (IgG2a) type. Furthermore, this inoculation suppressed immunoglobulin E (IgE) and IgG1 antibody responses to subsequent alum-precipitated Cry j 1 injections. Splenic T cells isolated from mice inoculated with pCACJ1 i.m. secreted interferon-gamma (IFN-gamma), but not interleukin (IL)-4, in vitro upon stimulation with Cry j 1 as well as with p277-288, a peptide corresponding to the T-cell epitope of Cry j 1. In contrast, inoculation ofBALB/c mice with pCACJ1 by gene gun injection caused response predominantly of the IgG1 type, and enhanced production of anti-Cry j 1 IgE antibodies to subsequent alum-precipitated Cry j 1 injections. Splenic T cells isolated from pCACJ1-innoculated mice by gene gun injection secreted both IFN-gamma and IL-4 in vitro, upon stimulation with Cry j 1 as well as with p277-288. These findings suggest that i.m. inoculation with pCACJ1 effectively elicits Cry j 1-specific T helper 1 (Th1)-type immune responses, resulting in inhibition of the IgE response to Cry j 1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:10:17