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Titolo:
Semi-synthetic Rab proteins as tools for studying intermolecular interactions
Autore:
Iakovenko, A; Rostkova, E; Merzlyak, E; Hillebrand, AM; Thoma, NH; Goody, RS; Alexandrov, K;
Indirizzi:
Max Planck Inst Mol Physiol, Dept Phys Biochem, D-44227 Dortmund, Germany Max Planck Inst Mol Physiol Dortmund Germany D-44227 7 Dortmund, Germany Romanian Acad, Inst Biochem, Bucharest 777000 17, Romania Romanian Acad Bucharest Romania 777000 17 , Bucharest 777000 17, Romania
Titolo Testata:
FEBS LETTERS
fascicolo: 2-3, volume: 468, anno: 2000,
pagine: 155 - 158
SICI:
0014-5793(20000225)468:2-3<155:SRPATF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERANYLGERANYL TRANSFERASE; BINDING; CYS;
Keywords:
intein; protein ligation; RabGGTase; Rab escort protein; Rab7;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Alexandrov, K Max Planck Inst Mol Physiol, Dept Phys Biochem, Otto Hahn Str 11, D-44227 Dortmund, Germany Max Planck Inst Mol Physiol Otto Hahn Str 11 Dortmund Germany D-44227
Citazione:
A. Iakovenko et al., "Semi-synthetic Rab proteins as tools for studying intermolecular interactions", FEBS LETTER, 468(2-3), 2000, pp. 155-158

Abstract

Rab GTPases play a key role in the regulation of membrane traffic. Posttranslational geranylgeranylation is critical for their biological activity and is conferred by a Rab geranylgeranyl transferase (RabGGTase). To study the interactions between Rab proteins and RabGGTase, we used in vitro ligation methodology to generate a fluorescent semi-synthetic Rab7 protein. The obtained protein was functionally active and was used to demonstrate a micromolar affinity interaction of Rab7 with the RabGGTase in the absence of Rab escort protein (REP). This finding is consistent with an earlier proposed model according to which RabGGTase possesses two independent weak binding sites for REP and Rab proteins. (C) 2000 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 08:57:10