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Titolo:
Calcium regulation of oxidative phosphorylation in rat skeletal muscle mitochondria
Autore:
Kavanagh, NI; Ainscow, EK; Brand, MD;
Indirizzi:
Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Univ Cambridge Cambridge England CB2 1QW hem, Cambridge CB2 1QW, England
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
fascicolo: 1-2, volume: 1457, anno: 2000,
pagine: 57 - 70
SICI:
0005-2728(20000224)1457:1-2<57:CROOPI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTATO-TUBER MITOCHONDRIA; DOWN ELASTICITY ANALYSIS; METABOLIC CONTROL ANALYSIS; MONTE-CARLO SIMULATION; PROTON LEAK; LIVER-MITOCHONDRIA; PYRUVATE-DEHYDROGENASE; CONTROL COEFFICIENTS; HEART MITOCHONDRIA; ENERGY-METABOLISM;
Keywords:
calcium; oxidative phosphorylation; top-down elasticity analysis; control analysis; regulation analysis; muscle mitochondria;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Brand, MD MRC, Dunn Human Nutr Unit, Hills Rd, Cambridge CB2 2XY, England MRC Hills Rd Cambridge England CB2 2XY mbridge CB2 2XY, England
Citazione:
N.I. Kavanagh et al., "Calcium regulation of oxidative phosphorylation in rat skeletal muscle mitochondria", BBA-BIOENER, 1457(1-2), 2000, pp. 57-70

Abstract

Activation of oxidative phosphorylation by physiological levels of calciumin mitochondria from rat skeletal muscle was analysed using top-down elasticity and regulation analysis. Oxidative phosphorylation was conceptually divided into three subsystems (substrate oxidation, proton leak and phosphorylation) connected by the membrane potential or the protonmotive force. Calcium directly activated the phosphorylation subsystem and (with sub-saturating 2-oxoglutarate) the substrate oxidation subsystem but had no effect on the proton leak kinetics. The response of mitochondria respiring on 2-oxoglutarate at two physiological concentrations of free calcium was quantified using control and regulation analysis. The partial integrated response coefficients showed that direct stimulation of substrate oxidation contributed 86% of the effect of calcium on state 3 oxygen consumption, and direct activation of the phosphorylation reactions caused 37% of the increase in phosphorylation flux. Calcium directly activated phosphorylation more strongly than substrate oxidation (78% compared to 45%) to achieve homeostasis of mitochondrial membrane potential during large increases in flux. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:40:13