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Titolo:
Mechanisms of cGMP-dependent mesangial-cell relaxation: a role for myosin light-chain phosphatase activation
Autore:
Torrecillas, G; Diez-Marques, ML; Garcia-Escribano, C; Bosch, RJ; Rodriguez-Puyol, D; Rodriguez-Puyol, M;
Indirizzi:
Univ Alcala de Henares, Dept Physiol, Madrid 28871, Spain Univ Alcala de Henares Madrid Spain 28871 t Physiol, Madrid 28871, Spain Univ Alcala de Henares, Dept Med, Madrid 28871, Spain Univ Alcala de Henares Madrid Spain 28871 Dept Med, Madrid 28871, Spain Hosp Principe Asturias, Nephrol Sect, Madrid 28805, Spain Hosp Principe Asturias Madrid Spain 28805 hrol Sect, Madrid 28805, Spain
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 346, anno: 2000,
parte:, 1
pagine: 217 - 222
SICI:
0264-6021(20000215)346:<217:MOCMRA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; CYCLIC-GMP; CONTRACTION; CALCIUM; VASOPRESSIN; SYSTEM; SIGNAL;
Keywords:
calyculin A; cyclic GMP; dibutyryl cGMP; myosin light-chain kinase; myosin light-chain phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Rodriguez-Puyol, M Univ Alcala de Henares, Dept Physiol, Carretera Madrid Barcelona Km 33-6, Madrid 28871, Spain Univ Alcala de Henares Carretera Madrid Barcelona Km 33-6 Madrid Spain 28871
Citazione:
G. Torrecillas et al., "Mechanisms of cGMP-dependent mesangial-cell relaxation: a role for myosin light-chain phosphatase activation", BIOCHEM J, 346, 2000, pp. 217-222

Abstract

Although the cGMP-dependent relaxation of contractile cells seems to depend on the ability of the cyclic nucleotide to interfere with intracellular calcium, this does not appear to be the only mechanism involved. The presentexperiments were designed to analyse alternative mechanisms, trying to test the hypothesis that cGMP could relax rat mesangial cells by activating myosin light-chain phosphatase (MLC-PP), with the subsequent dephosphorylation of myosin light chain (MLC). The effect of a cGMP analogue, dibutyryl cGMP (dbcGMP), on angiotensin II-(AII) and PMA-induced MLC phosphorylation (MLCP) was tested, in the presence of calyculin A (CA), an inhibitor of MLC-PP. MLCP was measured, after cell labelling with P-32, by immunoprecipitation. dbcGMP prevented the increased MLCP induced by AII or PMA, and this inhibition was blocked by CA. dbcGMP also increased the MLC dephosphorylation observed in cells incubated with AII and in which MLC kinase and protein kinase C activities were blocked. The AII-elicited increased intracellular calcium concentration was only partially inhibited by dbcGMP. These results suggest that the cGMP-induced mesangial-cell relaxation could be due, at leastpartially, to the stimulation of MLC-PP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:38:35