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Titolo:
Expression of 5-lipoxygenase by human colorectal carcinoma Caco-2 cells during butyrate-induced cell differentiation
Autore:
Wachtershauser, A; Steinhilber, D; Loitsch, SM; Stein, J;
Indirizzi:
Univ Frankfurt, Med Klin 2, Dept Med 2, D-60590 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60590 d 2, D-60590 Frankfurt, Germany Univ Frankfurt, Inst Pharmaceut Chem, D-60590 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60590 hem, D-60590 Frankfurt, Germany
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 268, anno: 2000,
pagine: 778 - 783
SICI:
0006-291X(20000224)268:3<778:EO5BHC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMING-GROWTH-FACTOR; HUMAN COLON-CANCER; ARACHIDONIC-ACID; FACTOR-BETA; GENE-EXPRESSION; SERUM FACTOR; HL-60 CELLS; INDUCTION; APOPTOSIS; 15-LIPOXYGENASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Stein, J Univ Frankfurt, Med Klin 2, Dept Med 2, Theodor Stern Kai 7, D-60590 Frankfurt, Germany Univ Frankfurt Theodor Stern Kai 7 Frankfurt Germany D-60590 any
Citazione:
A. Wachtershauser et al., "Expression of 5-lipoxygenase by human colorectal carcinoma Caco-2 cells during butyrate-induced cell differentiation", BIOC BIOP R, 268(3), 2000, pp. 778-783

Abstract

Butyrate, a short-chain fatty acid, modulates proliferation and differentiation of normal and neoplastic colonocytes. We examined the expression of B-Lipoxygenase (5-LO) and its metabolites in human colorectal carcinoma (Caco-2) cells, exposed to differentiation-inducing doses-of butyrate. Treatment with butyrate significantly increased 5-lipoxygenase mRNA and protein in comparison to nontreated cells. Cyclooxygenases (COX)-1 and COX-2 mRNA werenot significantly influenced by the treatment. However, 5-LO activity, lowin nontreated cells, increased only minimally after butyrate, and its metabolic product (5-HETE) was detectable neither in control nor in treated cells. In contrast, 15-HETE (a product of 15-LO, which is also upregulated by butyrate) rose significantly, We conclude that, although being overexpressed by butyrate on mRNA and protein level, 5-LO remains inactive in differentiating Caco-2 cells. This is likely to be due either to some associated actions of butyrate, or to 5-LO-inhibition by 15-HETE, concomitantly induced by butyrate treatment. (C) 2000 Academic Press.

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Documento generato il 24/09/20 alle ore 04:04:34