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Titolo:
Calcium regulation by skeletal muscle membranes of horses with recurrent exertional rhabdomyolysis
Autore:
Ward, TL; Valberg, SJ; Gallant, EM; Mickelson, JR;
Indirizzi:
Univ Minnesota, Coll Vet Med, Dept Vet Pathobiol, St Paul, MN 55108 USA Univ Minnesota St Paul MN USA 55108 Vet Pathobiol, St Paul, MN 55108 USA Univ Minnesota, Coll Vet Med, Dept Clin & Populat Sci, St Paul, MN 55108 USA Univ Minnesota St Paul MN USA 55108 & Populat Sci, St Paul, MN 55108 USA
Titolo Testata:
AMERICAN JOURNAL OF VETERINARY RESEARCH
fascicolo: 3, volume: 61, anno: 2000,
pagine: 242 - 247
SICI:
0002-9645(200003)61:3<242:CRBSMM>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC INTERMITTENT RHABDOMYOLYSIS; CA2+ RELEASE CHANNELS; MALIGNANT-HYPERTHERMIA; RYANODINE RECEPTOR; TWITCH CHARACTERISTICS; MUTATION; DISEASE; THRESHOLD; PIGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Mickelson, JR Univ Minnesota, Coll Vet Med, Dept Vet Pathobiol, St Paul, MN 55108 USA Univ Minnesota St Paul MN USA 55108 , St Paul, MN 55108 USA
Citazione:
T.L. Ward et al., "Calcium regulation by skeletal muscle membranes of horses with recurrent exertional rhabdomyolysis", AM J VET RE, 61(3), 2000, pp. 242-247

Abstract

Objective-To determine whether an alteration in calcium regulation by skeletal muscle sarcoplasmic reticulum, similar to known defects that cause malignant hyperthermia (MH), could be identified in membrane vesicles isolatedfrom the muscles of Thoroughbreds with recurrent exertional rhabdomyolysis(RER). Sample Population-Muscle biopsy specimens from 6 Thoroughbreds with RER and 6 healthy (control) horses. Procedures-RER was diagnosed on the basis of a history of > 3 episodes of exertional rhabdomyolysis confirmed by increases in serum creatine kinase (CK) activity. Skeletal muscle membrane vesicles, prepared by differential centrifugation of muscle tissue homogenates obtained from the horses, were characterized for sarcoplasmic reticulum (SR) activities, including the Ca2release rate for the ryanodine receptor-Ca2+ release channel, [H-3]ryanodine binding activities, and rate of SR Ca2+-ATPase activity and its activation by Ca2+. Results-Time course of SR Ca2+-induced Ca2+ release and [H-3]ryanodine binding to the ryanodine receptor after incubation with varying concentrationsof ryanodine, caffeine, and ionized calcium did not differ between muscle membranes obtained from control and RER horses. Furthermore, the maximal rate of SR Ca2+-ATPase activity and its affinity for Ca2+ did not differ between muscle membranes from control horses and horses with RER. Conclusions and Clinical Relevance-Despite clinical and physiologic similarities between RER and MH, we concluded that RER in Thoroughbreds does not resemble the SR ryanodine receptor defect responsible for MH and may represent a novel defect in muscle excitation-contraction coupling, calcium regulation, or contractility.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 17:08:48