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Titolo:
Characterisation of PCV-2 isolates from Spain, Germany and France
Autore:
Mankertz, A; Domingo, M; Folch, JM; LeCann, P; Jestin, A; Segales, J; Chmielewicz, B; Plana-Duran, J; Soike, D;
Indirizzi:
Robert Koch Inst, D-13353 Berlin, Germany Robert Koch Inst Berlin Germany D-13353 ch Inst, D-13353 Berlin, Germany Univ Autonoma Barcelona, Fac Vet, E-08193 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08193 E-08193 Barcelona, Spain AFSSA, Mol Biol Lab, F-22440 Ploufragan, France AFSSA Ploufragan France F-22440 Mol Biol Lab, F-22440 Ploufragan, France Ft Dodge Vet, Vall De Bianya 17813, Girona, Spain Ft Dodge Vet Vall De Bianya Girona Spain 17813 ianya 17813, Girona, Spain Staatliches Vet & Lebensmitteluntersuchungsamt, D-14469 Potsdam, Germany Staatliches Vet & Lebensmitteluntersuchungsamt Potsdam Germany D-14469 y
Titolo Testata:
VIRUS RESEARCH
fascicolo: 1, volume: 66, anno: 2000,
pagine: 65 - 77
SICI:
0168-1702(200001)66:1<65:COPIFS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTISYSTEMIC WASTING SYNDROME; PUTATIVE REPLICASE GENE; PORCINE CIRCOVIRUS; NUCLEOTIDE-SEQUENCE; PLANT CIRCOVIRUSES; VIRUS-DNA; PIGS; DISEASE; GEMINIVIRUSES; AFFINITIES;
Keywords:
porcine circovirus; postweaning multisystemic wasting syndrome (PMWS); Circoviridae; genomic sequence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Mankertz, A Robert Koch Inst, Nordufer 20, D-13353 Berlin, Germany Robert Koch Inst Nordufer 20 Berlin Germany D-13353 , Germany
Citazione:
A. Mankertz et al., "Characterisation of PCV-2 isolates from Spain, Germany and France", VIRUS RES, 66(1), 2000, pp. 65-77

Abstract

The new isolated circovirus variant PCV-2 is discussed to be the etiological agent of a new emerging swine disease with a variable morbidity and highlethality, postweaning multisystemic wasting syndrome (PMWS). PMWS has been diagnosed in North America and West Europe. Clinical signs include dyspnea, loss of weight, lymph node enlargement and lymphocyte depletion in lymphoid tissues. This report describes the characterisation of PCV-2 isolates from animals affected with PMWS from Germany, Spain and France. We could demonstrate the presence of circovirus by electron microscope, in situ hybridisation and PCR. PCR revealed incidence of PCV-2 in many tissues of one infected animal with the exception of heart and nervous system. The phylogenetic analysis of all PCV-2 isolates yet published in the database, showed relationship of isolates from Spain, Germany and France, with three sequences from Canada determined recently and two isolates from Taiwan, while other North American sequences,display a separate cluster. PCR screening of randomly collected organ samples from German pigs not affected with PMWS, revealeda rate of infection with PCV-1 of 5% and with PCV-2 of 26.8%, while blood samples showed a lower incidence. (C) 2000 Elsevier Science B.V. All rightsreserved.

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Documento generato il 05/04/20 alle ore 19:37:37