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Titolo:
Quality controls for bovine viral diarrhea virus-free IVF embryos
Autore:
Stringfellow, DA; Riddell, KP; Galik, PK; Damiani, P; Bishop, MD; Wright, JC;
Indirizzi:
Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA Auburn Univ Auburn AL USA36849 Univ, Coll Vet Med, Auburn, AL 36849 USA Infigen Inc, Deforest, WI 53531 USA Infigen Inc Deforest WI USA 53531Infigen Inc, Deforest, WI 53531 USA
Titolo Testata:
THERIOGENOLOGY
fascicolo: 3, volume: 53, anno: 2000,
pagine: 827 - 839
SICI:
0093-691X(200002)53:3<827:QCFBVD>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO FERTILIZATION; FOLLICULAR EPITHELIAL-CELLS; MONOCLONAL-ANTIBODIES; NONCYTOPATHIC STRAIN; ANTIGENIC DIVERSITY; TRYPSIN TREATMENT; CONTAMINATION; ASSOCIATION; OOCYTES; SYSTEM;
Keywords:
IVF; bovine embryos; bovine viral diarrhea virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Stringfellow, DA Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 ed, Auburn, AL 36849 USA
Citazione:
D.A. Stringfellow et al., "Quality controls for bovine viral diarrhea virus-free IVF embryos", THERIOGENOL, 53(3), 2000, pp. 827-839

Abstract

Introduction of bovine viral diarrhea virus (BVDV) with cumulus-oocyte-complexes (COCs) from the abattoir is a concern in the production of bovine embryos in vitro. Further, International Embryo Transfer Society (IETS) guidelines for washing and trypsin treatment of invivo-derived bovine embryos ensure freedom from a variety of pathogens, but these procedures appear to beless effective when applied to IVF embryos. In this study, COCs were exposed to virus prior to IVM, IVF and IVC. Then, virus isolations from cumulus cells and washed or trypsin-treated nonfertile and degenerated ova were evaluated as quality controls for IVF embryo production The effect of BVDV on rates of cleavage and development was also examined. All media were analyzed prior to the study for anti-BVDV antibody. Two approximately equal groupsof COCs from abattoir-origin ovaries were washed and incubated for 1 h in minimum essential medium (MEM) with 10% equine serum. One group was incubated in 10(7) cell culture infective doses (50% endpoint) of BVDV for 1 h, while the other was incubated without virus. Subsequently, the groups were processed separately with cumulus cells, which were present throughout IVM, IVF and IVC. Cleavage was evaluated at 4 d and development to morulae and blastocysts at 7 d of IVC. After IVC, groups of nonfertile and degenerated ova or morulae and blastocysts were washed or trypsin-treated, sonicated and assayed for virus. Cumulus cells collected at 4 and 7 d were also assayed for virus. Anti-BVDV antibody was found in serum used in IVM and IVC but notin other media. A total of 1,656 unexposed COCs was used to produce 1,284 cleaved embryos (78%), 960 embryos greater than or equal to 5 cells (58%), and 194 morulae and blastocysts (12%). A total of 1,820 virus-exposed COCs was used to produce 1,350 cleaved embryos (74%), 987 embryos greater than or equal to 5 cells (54%), and 161 morulae and blastocysts (9%). Rates of cleavage (P=0.021), cleavage to greater than or equal to 5 cells (P=0.026) and development to morula and blastocyst (P=0.005) were lower in the virus-exposed group (Chi-square test for heterogeneity). No virus was isolated fromany samples from the unexposed group. For the exposed group, virus was always isolated from 4- and 7-d cumulus cells, from all washed nonfertile and degenerated ova (n=40) and morulae and blastocysts (n=57) and from all trypsin-treated nonfertile and degenerated ova (n=80) and morulae and blastocysts (n=91). Thus, virus persisted in the system despite the presence of neutralizing antibody in IVM and IVC media, and both washing and trypsin treatment were ineffective for removal of the virus. Presence of virus in 4- and 7-d cumulus cells as well as in nonfertile and degenerated ova were good indicators of virus being associated with morulae and blastocysts. (C) 2000 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:50:20