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Titolo:
Structure of D-glyceraldehyde-3-phosphate dehydrogenase from Palinurus versicolor in a tetragonal crystal form
Autore:
Shen, YQ; Song, SY; Lin, ZJ;
Indirizzi:
Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China Chinese Acad Sci Beijing Peoples R China 100101 100101, Peoples R China
Titolo Testata:
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
fascicolo: 1, volume: 43, anno: 2000,
pagine: 96 - 104
SICI:
1006-9305(200002)43:1<96:SODDFP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOLO-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; RESOLUTION;
Keywords:
D-glyceraldehyde-3-phosphate dehydrogenase; allosteric enzyme and crystal structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Lin, ZJ Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China Chinese Acad Sci Beijing Peoples R China 100101 Peoples R China
Citazione:
Y.Q. Shen et al., "Structure of D-glyceraldehyde-3-phosphate dehydrogenase from Palinurus versicolor in a tetragonal crystal form", SCI CHINA C, 43(1), 2000, pp. 96-104

Abstract

D-glyceraldehyde-3-phosphate dehydrogenase (holo-GAPDH) from Palinurus versicolor was crystallized in a novel crystal form by the method of sitting-drop vapor diffusion. The crystals have space group P42(1)2, cell parametersa=15.49 nm, c=8.03 nm and two subunits per asymmetric unit. The crystal structure at 0.34 nm was determined by the molecular replacement method. The final model has crystallographic R-free and R factors of 0.274 and 0.262, and r.m.s. deviations of 0.002 nm for bond lengths and 2.33 degrees for bondangles. The two subunits in asymmetric unit are similar to each other not only in the three-dimensional structure, but also in average temperature factors. This result demonstrates that the obvious difference in average temperature factors for the different subunits in C2 crystal form reported previously may be attributed to the different crystallographic environments of the subunits, This further supports that holo-GAPDH has a good 222 molecular symmetry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 14:58:19