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Titolo:
Effects of intraluminal trypsin and bile on the exocrine and endocrine pancreas after pancreaticobiliary diversion and biliodigestive shunt
Autore:
Ohlsson, B; Yusa, T; Rehfeld, JF; Lundquist, I; Ihse, I; Axelson, J;
Indirizzi:
Univ Lund, Dept Surg, S-22100 Lund, Sweden Univ Lund Lund Sweden S-22100Univ Lund, Dept Surg, S-22100 Lund, Sweden Univ Lund, Dept Pharmacol, S-22100 Lund, Sweden Univ Lund Lund Sweden S-22100 Lund, Dept Pharmacol, S-22100 Lund, Sweden Rigshosp, Dept Clin Biochem, DK-2100 Copenhagen, Denmark Rigshosp Copenhagen Denmark DK-2100 Biochem, DK-2100 Copenhagen, Denmark
Titolo Testata:
PANCREAS
fascicolo: 2, volume: 20, anno: 2000,
pagine: 170 - 176
SICI:
0885-3177(200003)20:2<170:EOITAB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYME SECRETION; CHOLECYSTOKININ RELEASE; FEEDBACK-REGULATION; CONSCIOUS RATS; PLASMA; INFUSION; HUMANS; GROWTH; RADIOIMMUNOASSAY; HYPERCCKEMIA;
Keywords:
bile; biliodigestive shunt; cholecystokinin; pancreas; pancreaticobiliary diversion; trophic effects; trypsin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Axelson, J Univ Hosp, MAS, Dept Surg, SE-20502 Malmo, Sweden Univ Hosp Malmo Sweden SE-20502 Surg, SE-20502 Malmo, Sweden
Citazione:
B. Ohlsson et al., "Effects of intraluminal trypsin and bile on the exocrine and endocrine pancreas after pancreaticobiliary diversion and biliodigestive shunt", PANCREAS, 20(2), 2000, pp. 170-176

Abstract

Pancreaticobiliary diversion (PBD) and biliodigestive shunt (BDS) cause long-standing hypercholecystokininemia followed by pancreatic hyperplasia. These changes have been suggested to be due to the lack of intraluminal trypsin and bile, respectively, in the upper small intestine. The aim of these experiments was to study the effect of restoration of intraluminal trypsin and bile on plasma levels of cholecystokinin (CCK) and the changes found in exocrine and endocrine pancreas after PBD and BDS. Male Sprague-Dawley ratswere used. PBD was done in 16 rats, eight of which had trypsin dissolved in 50 mM sodium bicarbonate (SB), and eight had SE only by gastric intubation twice daily. BDS was done in another 16 rats, eight of which had bile dissolved in SE, and eight had SE in a similar manner. Sham-operated rats had SE and served as controls. After 4 weeks, the rats were killed, and the concentrations of circulating CCK, gastrin, glucose, glucagon, and insulin were determined. The pancreas was removed, weighed, and analyzed for contents of water, protein, and DNA. Ln another study, PBD-operated rats got trypsinin varying dosages or trypsin and taurocholate in combination for 2 weeks before death. The concentrations of plasma CCK and glucagon were elevated after both PBD and BDS, PBD decreased the concentration of gastrin in plasma. PBD caused an increase of pancreatic weight and the contents of protein and DNA. Trypsin substitution to PBD-operated rats did not affect plasma CCKor glucagon levels, but the PBD-induced increases in weight and DNA content were counteracted by trypsin. Higher dosages of trypsin did not further influence the effects seen after PBD. Pancreatic weight and DNA content wereincreased after BDS. Bile administration completely abolished the increasein plasma CCK and glucagon, as well as the gain in pancreatic weight, and reduced the increase in pancreatic DNA. Substitution with bile to BDS-operated rats abolished the increase in the plasma levels of CCK and Glucagon, as well as the trophic effects on the pancreas. Trypsin substitution to PBD-operated rats partly reversed the trophic effects on the pancreas but not the hormonal changes in plasma. Thus the trophic effects on the pancreas exerted by BDS seem to be dependent on the lack of bile in the upper small intestine, whereas the effects of PBD only partly are a consequence of the absence of intraluminal trypsin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 18:04:34