Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Repression of the integrated papillomavirus E6/E7 promoter is required forgrowth suppression of cervical cancer cells
Autore:
Francis, DA; Schmid, SI; Howley, PM;
Indirizzi:
Harvard Med Sch, Dept Pathol, Boston, MA 02115 USA Harvard Med Sch BostonMA USA 02115 ch, Dept Pathol, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 6, volume: 74, anno: 2000,
pagine: 2679 - 2686
SICI:
0022-538X(200003)74:6<2679:ROTIPE>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
E2 TRANSACTIVATION DOMAIN; HPV18 REGULATORY REGION; GENE-PRODUCT; HUMAN KERATINOCYTES; TYPE-16 DNA; FUNCTIONAL INTERACTION; TRANSCRIPTION FACTORS; EPITHELIAL-CELLS; TRANS-ACTIVATION; CARCINOMA CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Howley, PM Harvard Med Sch, Dept Pathol, 200 Longwood Ave, Boston, MA 02115 USA Harvard Med Sch 200 Longwood Ave Boston MA USA 02115 02115 USA
Citazione:
D.A. Francis et al., "Repression of the integrated papillomavirus E6/E7 promoter is required forgrowth suppression of cervical cancer cells", J VIROLOGY, 74(6), 2000, pp. 2679-2686

Abstract

The human papillomavirus (HPV) E2 protein is an important regulator of viral E6 and E7 gene expression. E2 can repress the viral promoter for E6 and E7 expression as well as block progression of the cell cycle in cancer cells harboring the DNA of "high-risk" HPV types. Although the phenomenon of E2-mediated growth arrest of HeLa cells and other HPV-positive cancer cells has been well documented, the specific mechanism by which E2 affects cellular proliferation has not yet been elucidated. Here, we show that bovine papillomavirus (BPV) E2-induced growth arrest of HeLa cells requires the repression of the E6 and E7 promoter. This repression is specific for E2TA and not E2TR, a BPV E2 variant that lacks the N-terminal transactivation domain. We demonstrate that expression of HPV16 E6 and E7 from a heterologous promoter that is not regulated by E2 rescues HeLa cells from E2-mediated growth arrest. Our data indicate that the pathway of E2-mediated growth arrest of HeLa cells requires repression of E6 and E7 expression through an activity specified by the transactivation domain of E2TA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:43:10