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Titolo:
Extraction and assay of ferulic acid esterase from malted barley
Autore:
Humberstone, FJ; Briggs, DE;
Indirizzi:
Univ Birmingham, Sch Biochem, Birmingham Malting & Brewing Grp, BirminghamB15 2TT, W Midlands, England Univ Birmingham Birmingham W Midlands EnglandB15 2TT W Midlands, England
Titolo Testata:
JOURNAL OF THE INSTITUTE OF BREWING
fascicolo: 1, volume: 106, anno: 2000,
pagine: 21 - 29
SICI:
0046-9750(200001/02)106:1<21:EAAOFA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARA-COUMAROYL ESTERASE; PLANT-CELL WALLS; ASPERGILLUS-NIGER; PHENOLIC-ACIDS; STREPTOMYCES-OLIVOCHROMOGENES; SACCHAROMYCES-CEREVISIAE; VOLATILE PHENOLS; DIFERULIC ACID; WHEAT BRAN; RELEASE;
Keywords:
barley malt; ferulic acid esterase; extraction; assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Humberstone, FJ Univ Birmingham, Sch Biochem, Birmingham Malting & BrewingGrp, POB 363, Birmingham B15 2TT, W Midlands, England Univ Birmingham POB 363 Birmingham W Midlands England B15 2TT
Citazione:
F.J. Humberstone e D.E. Briggs, "Extraction and assay of ferulic acid esterase from malted barley", J I BREWING, 106(1), 2000, pp. 21-29

Abstract

Ferulic acid esterase activity was detected in extracts of barley malt using an assay employing a novel artificial substrate, mono-feruloyl glycerol. Mono-fercrloyl glycerol has been synthesized and analysed to determine itsdegree of substitution and purity. It consists of a mixture of the two isomers 1-feruloyl glycerol and 2-feruloyl glycerol. The extraction of ferulicacid esterase and its assay conditions have been optimised. The presence of both a detergent and reduced glutathione in the extraction medium increased the amount of enzyme extracted. A pH of 7.5 was optimal for enzyme activity. The enzyme in solution was only stable up to 30 degrees C. The crude extract containing the enzyme released free ferulic acid from both soluble and insoluble cell wall materials. After extraction of the soluble enzyme, insoluble enzyme, capable of releasing free ferulic acid from feruloyl glycerol, was detected in the residual grain solids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 13:12:06