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Titolo:
Inhibition by intracellular Mg2+ of recombinant N-methyl-D-aspartate receptors expressed in Chinese hamster ovary cells
Autore:
Smerin, YL; Aizenman, E; Johnson, JW;
Indirizzi:
Univ Pittsburgh, Dept Neurosci, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 eurosci, Pittsburgh, PA 15260 USA Univ Pittsburgh, Sch Med, Dept Neurobiol, Pittsburgh, PA USA Univ Pittsburgh Pittsburgh PA USA ed, Dept Neurobiol, Pittsburgh, PA USA
Titolo Testata:
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
fascicolo: 3, volume: 292, anno: 2000,
pagine: 1104 - 1110
SICI:
0022-3565(200003)292:3<1104:IBIMOR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SINGLE-CHANNEL PROPERTIES; MOUSE CENTRAL NEURONS; CULTURED RAT NEURONS; NMDA RECEPTORS; XENOPUS-OOCYTES; SUBUNIT COMPOSITION; ACTIVATED CHANNELS; CORTICAL-NEURONS; NR1 SUBUNIT; BLOCK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Johnson, JW Univ Pittsburgh, Dept Neurosci, 446 Crawford Hall, Pittsburgh,PA 15260 USA Univ Pittsburgh 446 Crawford Hall Pittsburgh PA USA 15260 USA
Citazione:
Y.L. Smerin et al., "Inhibition by intracellular Mg2+ of recombinant N-methyl-D-aspartate receptors expressed in Chinese hamster ovary cells", J PHARM EXP, 292(3), 2000, pp. 1104-1110

Abstract

Intracellular Mg2+ (Mg-i(2+)) inhibits the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors in cultured cortical neurons. To examine the effects of Mg-i(2+) on recombinant NMDA receptors composed of subunit combinations found in cortical neurons, we expressed heteromeric receptors composed of NR1/NR2A and of NR1/NR2B subunits in Chinese hamster ovary (CHO) cells. We recorded whole-cell currents from the recombinant receptors in the absence and presence of Mg-i(2+). The voltage dependence of control (0 Mg-i(2+)) NMDA-activated currents obtained from CHO cells transfected with NR1/NR2A and with NR1/NR2B receptors showed outward rectification, a property thathas been observed previously in native cortical NMDA receptors. The magnitude and voltage dependence of inhibition by Mg-i(2+) of NMDA-activated currents were similar in CHO cells transfected with NR1/NR2A receptors, CHO cells transfected with NR1/NR2B receptors, and in cultured neurons expressing native NMDA receptors. These observations suggest that Mg-i(2+) has uniformeffects on the native NMDA receptors expressed in cortical neurons. Furthermore, inhibition by Mg-i(2+) must not depend on intracellular factors or post-translational receptor modifications that are specific to neurons. Finally, the results indicate that the previously observed differences between whole-cell and outside-out patch measurements of Mg-i(2+) inhibition could not result from poor control of voltage or Mg-i(2+) concentration in the dendrites of neurons. The most likely alternative explanation is that patch excision causes an alteration in NMDA receptors that results in more effective inhibition by Mg-i(2+).

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Documento generato il 30/11/20 alle ore 17:06:11