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Titolo:
Quantitation of in vivo measurements with carbon fiber microelectrodes
Autore:
Logman, MJ; Budygin, EA; Gainetdinov, RR; Wightman, RM;
Indirizzi:
Univ N Carolina, Curriculum Neurobiol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 obiol, Chapel Hill, NC 27599 USA Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 Chem, Chapel Hill, NC 27599 USA Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Labs, Dept Cell Biol, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 2, volume: 95, anno: 2000,
pagine: 95 - 102
SICI:
0165-0270(20000215)95:2<95:QOIVMW>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIAN FOREBRAIN-BUNDLE; IN-VIVO; CYCLIC VOLTAMMETRY; NUCLEUS-ACCUMBENS; CAUDATE-NUCLEUS; ELECTRICAL-STIMULATION; INVIVO VOLTAMMETRY; RAT STRIATUM; DOPAMINE; MICRODIALYSIS;
Keywords:
fast-scan cyclic voltammetry; microdialysis; acetaminophen; striatum; carbon fiber microelectrodes; pH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Logman, MJ Univ N Carolina, Curriculum Neurobiol, Venable Hall,CB 3290, Chapel Hill, NC 27599 USA Univ N Carolina Venable Hall,CB 3290 Chapel Hill NCUSA 27599 A
Citazione:
M.J. Logman et al., "Quantitation of in vivo measurements with carbon fiber microelectrodes", J NEUROSC M, 95(2), 2000, pp. 95-102

Abstract

Fast-scan cyclic voltammetry (FSCV) at carbon fiber disk microelectrodes and quantitative microdialysis were used to measure striatal concentration changes of N-acetyl-p-aminophenol (APAP, acetaminophen) following an intraperitoneal injection of 75 mg/kg APAP in rats. The goal of this work was to determine which in vitro calibration procedure, precalibration or postcalibration, gave the most accurate results when using carbon fiber microelectrodes in vivo. Voltammetric detection of APAP in vivo was complicated with normal electrodes by interference from pH changes. An electrode treatment was used to minimize electrode sensitivity to pH and this allowed successful APAP detection. In vitro calibrations of the treated carbon fiber disk microelectrodes before and after the in vivo experiment were used to calculate APAP concentration changes measured in vivo and compared to microdialysis results. The maximal striatal APAP concentration determined by microdialysis, adjusted for in vitro recovery, was 23.I mu M. The electrochemical results were approximately two times greater (postcalibration) or smaller (precalibration) than the microdialysis result. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:13:59