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Titolo:
Acute effects of interferon on estrogen receptor function do not involve the extracellular signal-regulated kinases p42(mapk) and p44(mapk)
Autore:
Flint, APF; Abayasekara, DRE; Wheeler-Jones, CPD; Riley, PR; Kaluz, S; Kaluzova, M; Sheldrick, ELR; Fisher, PA;
Indirizzi:
Univ Nottingham, Sch Biol Sci, Sutton Bonington LE12 5RD, Leics, England Univ Nottingham Sutton Bonington Leics England LE12 5RD D, Leics, England Univ London Royal Vet Coll, Reprod & Dev Grp, London, England Univ London Royal Vet Coll London England od & Dev Grp, London, England
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 2, volume: 20, anno: 2000,
pagine: 225 - 233
SICI:
1079-9907(200002)20:2<225:AEOIOE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; BREAST CANCER-CELLS; TYROSINE PHOSPHORYLATION; TRANSCRIPTIONAL ACTIVATION; INDEPENDENT ACTIVATION; COUPLED RECEPTORS; BETA-INTERFERON; MCF-7 CELLS; IN-VITRO; C-DELTA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Flint, APF Univ Nottingham, Sch Biol Sci, Sutton Bonington LE12 5RD, Leics, England Univ Nottingham Sutton Bonington Leics England LE12 5RD ngland
Citazione:
A.P.F. Flint et al., "Acute effects of interferon on estrogen receptor function do not involve the extracellular signal-regulated kinases p42(mapk) and p44(mapk)", J INTERF CY, 20(2), 2000, pp. 225-233

Abstract

Exposure to type I interferons (IFN) increased estrogen receptor (ER) ligand binding and induced protein kinase C (PKC) translocation within 30 min but had no effect on net incorporation of [(32)p] info ER in Madin Darby bovine kidney (MDBK) cells. Ligand binding was also increased within 30 min byphorbol ester and the protein phosphatase inhibitor okadaic acid. Mitogen-activated protein (MAP) kinase phosphorylation was initially inhibited between 2 and 30 min and subsequently activated between 30 and 60 min after treatment with IFN. The activatory response was blocked by the PKC inhibitor Ro 31-8220. Following transient transfection with an ERE-CAT reporter construct, IFN increased CAT expression after 6 h but decreased ER ligand binding, transcriptional activity and phosphorylation after 48 h, probably as a result of decreased ER concentrations, The results rule out rapid activation of ER ligand binding through phosphorylation at Ser(118) by MAP kinase because (1) the increase in ligand binding preceded activation of MAP kinase, and (2) IFN had no short-term effect on [P-32]incorporation or ER transcriptional activity. The rapid effect of LFN on ER ligand binding is postulated to reflect phosphorylation of the receptor at Tyr(537) by p56(lck), a member of the Src family of PKC-activated tyrosine kinases.

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Documento generato il 24/09/20 alle ore 05:20:06