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Titolo:
Lipopolysaccharide induces scavenger receptor a expression in mouse macrophages: A divergent response relative to human THP-1 monocyte/macrophages
Autore:
Fitzgerald, ML; Moore, KJ; Freeman, MW; Reed, GL;
Indirizzi:
Harvard Univ, Sch Publ Hlth, Cardiovasc Biol Lab, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ardiovasc Biol Lab, Boston, MA 02115 USA Harvard Univ, Sch Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 vard Univ, Sch Med, Boston, MA 02115 USA Harvard Univ, Sch Med, Massachusetts Gen Hosp, Lipid Metab Unit, Boston, MA 02114 USA Harvard Univ Boston MA USA 02114 , Lipid Metab Unit, Boston, MA 02114 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 5, volume: 164, anno: 2000,
pagine: 2692 - 2700
SICI:
0022-1767(20000301)164:5<2692:LISRAE>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HUMAN MONOCYTE-MACROPHAGES; NECROSIS-FACTOR-ALPHA; BACTERIAL-ENDOTOXIN; LIGAND-BINDING; CELL LINES; GENE; INHIBITION; SEQUENCES; ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Reed, GL Harvard Univ, Sch Publ Hlth, Cardiovasc Biol Lab, 677 Huntington Ave, Boston, MA 02115 USA Harvard Univ 677 Huntington Ave Boston MA USA 02115 MA 02115 USA
Citazione:
M.L. Fitzgerald et al., "Lipopolysaccharide induces scavenger receptor a expression in mouse macrophages: A divergent response relative to human THP-1 monocyte/macrophages", J IMMUNOL, 164(5), 2000, pp. 2692-2700

Abstract

Gene deletion studies indicate that the macrophage scavenger receptor A (SR-A) protects mice from LPS-induced endotoxemia. Paradoxically, cultured human monocyte-derived macrophages down-regulate SR-A expression when exposedto LPS. We found that human THP-1 monocyte/macrophages decrease SR-A expression in response to LPS independent of their differentiation status. In contrast, primary and elicited mouse peritoneal macrophages as well as the 5774A.1 and RAMr2664.7 mouse macrophage lines increase SR-A expression in response to LPS. Exposure to LPS caused J774A.1 and RAW264.7 cells to increaseSR-A transcripts by 3- and 5-fold, respectively. LPS caused a concomitant 3-fold increase in SR-A protein levels and increased cell membrane expression of the receptor, RAW264.7 cells increased SR-A transcript levels in response to LPS at concentrations as low as 1 ng/ml, and the response was saturated at 10 ng/ml, The LPS induction of SR-A transcripts required continual protein synthesis and began at 8 h, peaked by 16 h, and persisted for at least 48 h, LPS induction did not increase SR-A gene transcription or affect alternative transcript splicing, but mildly increased mature transcript stability and proceeded in the presence of actinomycin D. Finally, treatment of RAW264.7 cells with TNF-alpha did not induce SR-A transcript levels, indicating that a TNF-alpha autocrine/paracrine signaling mechanism alone is not sufficient to recapitulate the LPS induction of SR-A transcripts, The induction of SR-A expression by LPS-stimulated mouse macrophages is the opposite of the down-regulation of SR-A reported in human monocyte-derived macrophages and may have implications for the observed resistance mice show toward endotoxemia.

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Documento generato il 31/03/20 alle ore 15:50:35