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Titolo:
Semi-automatic liquid chromatographic analysis of olpadronate in urine andserum using derivatization with (9-fluorenylmethyl)chloroformate
Autore:
Sparidans, RW; den Hartigh, J; Cremers, S; Beijnen, JH; Vermeij, P;
Indirizzi:
Leiden Univ, Med Ctr, Dept Clin Pharm & Toxicol, NL-2300 RC Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RC NL-2300 RC Leiden, Netherlands Slotervaart Hosp, Dept Pharm & Pharmacol, NL-1066 EC Amsterdam, Netherlands Slotervaart Hosp Amsterdam Netherlands NL-1066 EC Amsterdam, Netherlands
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 738, anno: 2000,
pagine: 331 - 341
SICI:
1387-2273(20000211)738:2<331:SLCAOO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRECOLUMN DERIVATIZATION; FLUORESCENCE DETECTION; ELECTROCHEMICAL DETECTION; PLASMA; BISPHOSPHONATE; BONE; PAMIDRONATE; 1-NAPHTHYLISOTHIOCYANATE; AMINES; ASSAY;
Keywords:
derivatization; LC; olpadronate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Sparidans, RW Univ Utrecht, Fac Pharm, Dept Pharmaceut Anal, Sorbonnelaan 16, NL-3584 CAUtrecht, Netherlands Univ Utrecht Sorbonnelaan 16 Utrecht Netherlands NL-3584 CA
Citazione:
R.W. Sparidans et al., "Semi-automatic liquid chromatographic analysis of olpadronate in urine andserum using derivatization with (9-fluorenylmethyl)chloroformate", J CHROMAT B, 738(2), 2000, pp. 331-341

Abstract

The semi-automatic bioanalytical assays for olpadronate [(3-dimethylamino-1-hydroxypropylidene)bisphosphonate] involves a protein precipitation with trichloroacetic acid and a double co-precipitation with calcium phosphate for serum samples and a triple calcium co-precipitation for urine samples. These manual procedures are followed by an automated solid-phase extraction on a cation-exchange phase. The procedure is continued either directly, at high olpadronate levels in urine, or after off-line evaporation under nitrogen and reconstitution in water on the same robotic workstation. The continued automatic procedure comprehends derivatization with (9-fluorenylmethyl)chloroformate, ion-pair liquid-liquid extraction and ion-pair HPLC with fluorescence detection at 274/307 nm. The intra- and inter-day precisions for urine and serum samples are typically in the 5-8% range for different olpadronate concentrations [levels near the lower limit of quantification (LLQ) excluded]. The LLQ is 5 ng/ml olpadronate for a 2.5-ml urine sample and 10 ng/ml for a 1-ml serum sample, respectively. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:56:23