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Titolo:
Simultaneous separation of angiotensin and endothelin peptide families by high-performance liquid chromatography: application to the specific radioimmunoassay measurement of angiotensin II or endothelin-1 from tissue
Autore:
Naik, GOA; Moe, GW;
Indirizzi:
Univ Toronto, St Michaels Hosp, Toronto, ON M5B 1W8, Canada Univ Toronto Toronto ON Canada M5B 1W8 Hosp, Toronto, ON M5B 1W8, Canada Univ Toronto, Dept Med, Toronto, ON M5B 1W8, Canada Univ Toronto Toronto ON Canada M5B 1W8 t Med, Toronto, ON M5B 1W8, Canada
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 870, anno: 2000,
pagine: 349 - 361
Fonte:
ISI
Lingua:
ENG
Soggetto:
STATIONARY PHASES; QUANTITATION; PLASMA; HEART;
Keywords:
angiotensins; endothelins; peptides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Naik, GOA Univ Toronto, St Michaels Hosp, Toronto, ON M5B 1W8, Canada UnivToronto Toronto ON Canada M5B 1W8 onto, ON M5B 1W8, Canada
Citazione:
G.O.A. Naik e G.W. Moe, "Simultaneous separation of angiotensin and endothelin peptide families by high-performance liquid chromatography: application to the specific radioimmunoassay measurement of angiotensin II or endothelin-1 from tissue", J CHROMAT A, 870(1-2), 2000, pp. 349-361

Abstract

Currently available measurements of endogenous angiotensin II (ANG II) andendothelin-1 (ET-1) concentrations by radioimmunoassay (RIA) lack specificity to ANG II or ET-1. ANG II and ET-1 antibodies cross-react with immune-reactive angiotensin and endothelin family members, respectively. We have therefore developed an ion-pair reversed-phase high-performance liquid chromatography (HPLC) for simultaneously separating angiotensin and endothelin peptides and enhancing RIA specificity in the measurement of ANG II and ET-1. The developed HPLC separation was applied to canine myocardium extracts; ANG II or ET-1 fractions were collected and quantified by RIA. Elution timesfor both peptide families, ANG I, ANG LI, ANG III, ANG IV, ANG II(4-8), bET-1, ET-1, ET-2 and ET-3 were within 25 min. In normal canine myocardium from the right atrium, right ventricle, left atrium and left ventricle, ANG II concentrations were 39+/-11, 28+/-21, 31+/-11 and 21+/-8 fmol/g and ET-1 concentrations were 43+/-16, 42+/-19, 55+/-21 and 57+/-34 fmol/g (mean+/-SD, N=7), respectively. The combination of HPLC with RIA renders the measurement of ANG II or ET-I specific and convenient, and saves time. This HPLC separation may be applied to the specific measurement of other immune-reactive angiotensin and endothelin peptides. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 09/07/20 alle ore 12:56:11