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Titolo:
Colocalization of GP125/CD98 with tropomyosin isoforms at the cell-cell adhesion boundary
Autore:
Shishido, T; Ohkawa, M; Itoh, A; Enomoto, T; Hashimoto, Y; Masuko, T;
Indirizzi:
Tohoku Univ, Grad Sch Pharmaceut Sci, Mol Cell Biol Lab, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Ku, Sendai, Miyagi 9808578, Japan Sasaki Inst, Chiyoda Ku, Tokyo 1010062, Japan Sasaki Inst Tokyo Japan 1010062 i Inst, Chiyoda Ku, Tokyo 1010062, Japan
Titolo Testata:
JOURNAL OF BIOCHEMISTRY
fascicolo: 2, volume: 127, anno: 2000,
pagine: 253 - 261
SICI:
0021-924X(200002)127:2<253:COGWTI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; ANCHORAGE-INDEPENDENT GROWTH; MURINE MONOCLONAL-ANTIBODIES; MESSENGER-RNA LOCALIZATION; XENOPUS-LAEVIS OOCYTES; RAT-KIDNEY CELLS; SURFACE-ANTIGEN; HEAVY-CHAIN; ACTIVATED LYMPHOCYTES; TRANSFORMED-CELLS;
Keywords:
cell-cell adhesion; cytoskeleton; GP125/CD98; monoclonal antibody; tropomyosin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Shishido, T Tohoku Univ, Grad Sch Pharmaceut Sci, Mol Cell Biol Lab, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Miyagi 9808578, Japan
Citazione:
T. Shishido et al., "Colocalization of GP125/CD98 with tropomyosin isoforms at the cell-cell adhesion boundary", J BIOCHEM, 127(2), 2000, pp. 253-261

Abstract

Two monoclonal antibodies designated as 1F6 and 4B10 were obtained on screening for reactivities to CD98-associated molecules by sandwich-type enzyme-linked immunosorbent assaying using hybridoma culture supernatants as the solid phase, cell lysates as an antigen source, and a mixture of biotinylated antibodies to CD98HC as a detector. Flow cytometric analysis with microspheres in combination with 1F6, 4B10, and anti-CD98HC also indicated the association of antibody-defined antigen(s) with CD98, 1F6 and 4B10, stained fibrillate components in fixed and permeated cells but were not reactive with unfixed live cells, suggesting that epitopes reside in the cytoskeleton-associated structure in the intracellular region. Two-color immunostaining followed by confocal microscopy revealed the colocalization of the antigen with CD98 at the cell-cell adhesion boundary of HeLa cells, 1F6 detected proteins with relative molecular masses of 33,000 to 43,000 on immunoblotting analysis involving cell lysates of human and rat cell lines. Analysis with a purified tropomyosin specimen from rabbit skeletal muscle demonstrated that 1F6 and 4B10 recognize tropomyosin, Two-dimensional gel electrophoresis followed by immunoblotting analysis revealed that 1F6 recognizes various tropomyosin isoforms, These results indicated that CD98 physically associatesdirectly or indirectly with tropomyosin, and that this association is closely related to the cell-cell interaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:12:07